基于小分子多肽的新型口腔抑菌剂对菌斑生物膜的作用研究  

Effect of Novel Oral Bacteriostatic Agents Based on Antimicrobial Peptides on Oral Bacterial Biofilms

作　　者：闵祎凡 项勇刚[2] 胡梓秋 毛敏[1] 夏凌云[1] MIN Yifan;XIANG Yonggang;HU Ziqiu;MAO Min;XIA Lingyun(Department of Stomatology,Taihe Hospital,Hubei University of Medicine,Shiyan 442000,China;Department of Ophthalmology,Taihe Hospital,Hubei University of Medicine,Shiyan 442000,China)

机构地区：[1]湖北医药学院附属太和医院口腔医学中心,湖北十堰442000 [2]湖北医药学院附属太和医院眼科中心,湖北十堰442000

出　　处：《口腔医学研究》2023年第12期1050-1055,共6页Journal of Oral Science Research

基　　金：湖北省教育厅科学研究计划(编号:Q20222114);湖北省教育厅科学研究计划指导性项目(编号:B2022499);湖北医药学院2022年度教学研究项目(编号:2022017);湖北医药学院药护学院2022年度教学研究项目(编号:YHJ2022025)。

摘　　要：目的:在体外构建的菌斑生物膜模型上,进一步评价基于小分子多肽的新型口腔抑菌剂对菌斑生物膜的作用效果。方法:构建3 d龄菌斑生物膜模型,采用抗菌肽单独处理和联合使用的方法,通过激光共聚焦显微镜及结晶紫染色实验分析比较不同处理对菌斑生物膜杀菌及抑制生物膜形成的作用效果。结果:抗菌肽buCaTHL4B对成熟牙菌斑生物膜杀菌作用较强,杀菌率为(42.4±2.7)%。buCaTHL4B与不同浓度溶菌酶溶液及乙二胺四乙酸联合使用时杀菌率分别为[MIX 1:(49.2±1.8)%;MIX 2:(59.5±1.8)%;MIX 3:(59.2±1.7)%],均优于单独使用(P<0.01)。buCaTHL4B与浓度为10 mg/mL的溶菌酶溶液联合使用时具有更持久的杀菌效果,并能更显著地抑制生物膜形成。结论:基于小分子多肽buCaTHL4B复合溶液对菌斑生物膜有较好的杀菌及抑制生物膜形成的作用效果。Objective:To evaluate the effect of novel oral bacteriostatic agents based on antimicrobial peptides on oral bacterial biofilms by in vitro model.Methods:A 3-day-old oral bacterial biofilm model was constructed,and the effects of different contents on oral bacterial biofilm sterilization and inhibition of biofilm formation were analyzed by laser confocal microscopy and crystal violet staining experiment.Results:The antibacterial peptide buCaTHL4B had strong bactericidal ability against mature oral bacterial biofilms,and its sterilization rate was(42.4±2.7)%.When buCaTHL4B was used in combination with different concentrations of lysozyme solution and EDTA,the sterilization rates were[MIX 1:(49.2±1.8)%;MIX 2:(59.5±1.8)%and MIX 3:(59.2±1.7)%],respectively,which were better than those used alone(P<0.01).buCaTHL4B had a more durable bactericidal effect when combined with lysozyme at a concentration of 10 mg/mL.buCaTHL4B could inhibit biofilm formation more significantly when combined with lysozyme at a concentration of 10 mg/mL than buCaTHL4B alone.Conclusion:The mixed bacteriostatic solution based on small molecule polypeptide buCaTHL4B has good effect on the oral bacterial killing and formation inhibiting of biofilm.

关 键 词：抗菌肽 溶菌酶 乙二胺四乙酸 buCaTHL4B 口腔细菌生物膜 

分 类 号：R781[医药卫生—口腔医学]