次氯酸钠和EDTA预处理对釉质白斑树脂渗透效果影响的实验研究  

Effect of NaClO and EDTA Pretreatment on Resin Infiltration of Enamel White Spot Lesions:An in vitro Study

作　　者：倪恪 王琰 贺晶宇 李迎梅 马艳红 孟箭 孟庆飞[1,2,3] NI Ke;WANG Yan;HE Jingyu;LI Yingmei;MA Yanhong;MENG Jian;MENG Qingfei(Department of Stomatology,Xuzhou Clinical College,Xuzhou Medical University,Xuzhou 221000,China;Department of Stomatology,Xuzhou Central Hospital,Xuzhou 221000,China;School of Stomatology,Xuzhou Medical University,Xuzhou 221000,China)

机构地区：[1]徐州医科大学徐州临床学院口腔科,江苏徐州221000 [2]徐州市中心医院口腔科,江苏徐州221000 [3]徐州医科大学口腔医学院,江苏徐州221000

出　　处：《口腔医学研究》2023年第12期1056-1062,共7页Journal of Oral Science Research

基　　金：江苏省“六个一工程”拔尖人才(编号:LGY2020027);徐州市卫生健康委科技项目(编号:XWKYHT20220114)。

摘　　要：目的:比较不同预处理剂对釉质白斑表面孔隙暴露率、树脂渗透效果和表面显微硬度的影响。方法:96颗完整离体前磨牙在牙冠颊面正中切取4 mm×4 mm×2 mm釉质块。经体外脱矿3 d建立釉质白斑脱矿模型。将样本随机分为4组(n=24):A组为对照组;B组用5.25%NaClO预处理;C组用17%EDTA预处理;D组用5.25%NaClO+17%EDTA联合处理,所有样本预处理结束后均使用15%盐酸酸蚀处理。每组各8个样本观察计算釉质表面孔隙暴露面积和暴露率;每组另取8个样本经树脂渗透治疗后观察树脂渗透深度和渗透面积百分比;每组剩余8个样本测量树脂渗透前后釉质表面显微硬度值。结果:与A组相比,B、C、D组样本的釉质表面孔隙暴露面积、暴露率、树脂渗透深度、渗透面积百分比和树脂渗透后釉质表面显微硬度均显著提高(P<0.05);经17%EDTA或5.25%NaClO+17%EDTA处理后,C、D组脱矿釉质的显微硬度值明显低于A、B组(P<0.05)。结论:5.25%NaClO和17%EDTA预处理均可显著增加釉质孔隙暴露率,提高树脂渗透深度和表面显微硬度,但17%EDTA预处理会降低脱矿釉质的显微硬度。Objective:To compare the effect of different pretreatment agents on the pore exposure rate of the enamel surface,resin infiltration effectiveness,and surface microhardness of WSLs.Methods:A 4 mm×4 mm×2 mm enamel block from 96 sound premolars was cut in the center of the buccal surface of the teeth.The in vitro demineralization models of WSLs were established after placing the enamel blocks in the demineralization solution for 3 days.All samples were randomly divided into 4 groups(n=24):The samples in Group A given no pretreatment were control;Group B was pretreated with 5.25%NaClO solution;Group C was pretreated with 17%EDTA solution;Group D was pretreated with 5.25%NaClO and 17%EDTA solutions.All samples were etched with 15%hydrochloric acid after pretreatments.Eight samples of each group were used to calculate the exposure area and exposure rate of the pores in enamel surfaces.Another 8 samples were collected and used for evaluating the penetration depths and penetration area percentage after resin infiltration treatment of WSLs.The remaining 8 samples in each group were used to measure the surface microhardness of the enamel before and after resin infiltration.Results:Compared with group A,the exposed area and rate,resin penetration depth and percentage of penetration area,and surface microhardness of enamel in groups B,C,and D were significantly improved(P<0.05).After pretreated with 17%EDTA or 5.25%NaClO combined with 17%EDTA,the microhardness of demineralized enamel in groups C and D was significantly lower than that in groups A and B(P<0.05).Conclusion:Surface pretreatment with 5.25%NaClO or 17%EDTA can significantly increase enamel pore exposure rate,resin penetration depth,and surface microhardness,but pretreatment with 17%EDTA can reduce the microhardness of demineralized enamel.

关 键 词：釉质白斑 树脂渗透 预处理 次氯酸钠 乙二胺四乙酸 

分 类 号：R781.2[医药卫生—口腔医学]