运动相关基因纤维连接蛋白Ⅲ结构域蛋白5与乳腺癌的进展相关性研究  

Exercise-related gene FNDC5 and its association with breast cancer progression

作　　者：宋云蕾 罗燕 SONG Yun-lei;LUO Yan(Scientific Research Center,Gannan Medical University,Ganzhou,Jiangxi 341000)

机构地区：[1]赣南医学院科研中心,江西赣州341000

出　　处：《赣南医学院学报》2023年第10期1018-1028,共11页JOURNAL OF GANNAN MEDICAL UNIVERSITY

基　　金：江西省教育厅科学技术研究项目(GJJ211517,GJJ2201456)。

摘　　要：目的:探讨纤维连接蛋白Ⅲ结构域蛋白5(Fibronectin typeⅢdomain-containing protein 5,FNDC5)对乳腺癌进展的影响。方法:采用TIMER、Breast Cancer Gene-Expression Miner v4.9、Kaplan-Meier Plotter、cBioPortal、UALCAN、SurvivalMeth、DIANA-microT、miRWalk、miRTarBase、GSEA、STRING、R等方法,研究乳腺癌患者临床预后参数和生存数据与FNDC5的关系,以及FNDC5在乳腺癌患者中的表达机制。结果:与正常乳腺组织相比,FNDC5基因在乳腺癌中的表达明显降低。乳腺癌中,FNDC5表达水平与年龄、雌激素受体(Estrogen receptor,ER)阴性、孕激素受体(Progesterone receptor,PR)阴性、表皮生长因子受体-2(Human epidermal growth factor receptor 2,HER2)阳性、淋巴结阳性、组织学类型、SBR分级、三阴性和基底样状态均具有相关性,还可能与mir-4323和mir-4692的表达具有相关性。此外,FNDC5低表达与乳腺癌患者不良预后相关,FNDC5参与了与肿瘤转移进展相关的信号通路。结论:FNDC5在乳腺癌中表达下调,且其低表达与乳腺癌的不良预后相关,同时,其低表达可能由mir-4323和mir-4692参与调控。Objective:To investigate the influence of fibronectin typeⅢdomain-containing protein 5(FNDC5)on the progression of breast cancer.Methods:TIMER,Breast Cancer Gene-Expression Miner v4.9,Kaplan-Meier Plotter,Cbioportal,UALCAN,Survival Meth,DIANA-micro T,miRWalk,miRTarBase,GSEA,STRING and R were performed to explore the relationship between clinical prognostic parameters and survival data with FNDC5,and the mechanisms of the FNDC5 expression in breast cancer patients.Results:Compare to normal breast tissue,FNDC5 gene expression was significantly lower in breast cancer.The age,estrogen receptor(ER)negative,progesterone receptor(PR)status negative,epidermal growth factor receptor-2(HER2)positive,lymph node positive,histological types,Scarff-Bloom-Richardson(SBR)grade,triple-negative status and basal-like status were correlated with FNDC5 level,and FNDC5 level may also be correlated with the mir-4323 and mir-4692.Furthermore,the decreased FNDC5 expression correlated with worse overall survival.Besides that,FNDC5 also involved in the signaling pathway related to the tumor metastasis and progression.Conclusion:The expression of FNDC5 is down-regulated in breast cancer,and its low expression is associated with poor prognosis of breast cancer.Meanwhile,its low expression may be regulated by mir-4323 and mir-4692.

关 键 词：纤维连接蛋白Ⅲ结构域蛋白5 乳腺癌 微小核糖核酸 运动 

分 类 号：R739.9[医药卫生—肿瘤]