氧化还原敏感型基因元件增强酵母木质纤维素水解液抑制物胁迫耐受性  

Redox-sensitive Genetic Parts Improve the Tolerance of Yeast toLignocellulosic Hydrolysate Inhibitors

作　　者：王文韬 冯颀 刘晨光[1] 白凤武[1] 赵心清[1] WANG Wen-tao;FENG Qi;LIU Chen-guang;BAI Feng-wu;ZHAO Xin-qing(School of Life Sciences and Biotechnology,Shanghai Jiao Tong University,Shanghai 200240)

机构地区：[1]上海交通大学生命科学技术学院,上海200240

出　　处：《生物技术通报》2023年第11期360-372,共13页Biotechnology Bulletin

基　　金：国家自然科学基金项目(21978167);上海市自然科学基金项目(18ZR 1420700)。

摘　　要：纤维素乙醇作为一种清洁可再生的绿色能源,具有良好的应用前景。然而酿酒酵母利用木质纤维素原料生产乙醇的发酵过程易受多种抑制物胁迫的影响,因此提高其胁迫耐受性具有重要意义。本研究在细胞内设计了一种氧化还原敏感型基因元件,通过生物传感器Yap1感应胞内氧化还原状态,以调控抗胁迫基因智能表达。首先,分析了Yap1调控的天然内源启动子P_(TRR1)、P_(TRX2)和P_(MET16)对木质纤维素水解液中典型抑制物的响应强度。其次,根据不同胁迫种类组合相应启动子与抗胁迫的效益基因,构建氧化还原敏感型基因元件提高了酿酒酵母的胁迫耐受性。最后,将表现较好的基因元件GP-CTT和GP-ADH串联整合到一起构建了双基因元件系统,在5-HMF和H_(2)O_(2)双重胁迫下细胞的死亡率与野生型相比下降了69.6%。相较于单基因元件GP-CTT,双基因元件整合菌株的比生长速率、葡萄糖消耗速率和乙醇生产速率分别提高了64.2%、60.1%和58.9%,重组菌株过氧化氢酶的酶活力提高了40.2%。本研究通过理性设计氧化还原敏感型基因元件的遗传回路,强化胞内关键抗氧化酶和醛降解途径,系统地提高了酿酒酵母的胁迫耐受性,为动态地提高酵母鲁棒性提供了新的见解。Cellulosic ethanol,as a clean and renewable green energy,has promising application prospects.However,the fermentation process of Saccharomyces cerevisiae using lignocellulose to produce ethanol is susceptible to various stresses,thus improving the stress tolerance of fermenting microorganisms has become an essential topic in the field of biorefinery.In this work,we attempted to engineer cells to use the output of the internal redox-sensitive biosensor Yap1 to drive intracellular metabolic reactions(including ROS metabolism and aldehyde degradation),which will improve the tolerance of S.cerevisiae to reactive oxygen species and aldehyde inhibitors.Firstly,we analyzed the sequences of several native endogenous promoters(P_(TRR1),P_(TRX2),and P_(MET16))in S.cerevisiae that can be regulated by the redox-sensitive biosensor Yapl and their response strengths to typical inhibitors in lignocellulosic hydrolysates.Secondly,we designed redox-sensitive genetic parts in order to improve the stress resistance of S.cerevisiae by combining the promoters with corresponding resistance genes.Finally,we tried to integrate the better performing GP-CTT and GP-ADH in tandem to construct a dual-genetic parts system,which was named GP-AC.The integration greatly improved the ability of S.cerevisiae to cope with multiple complex stresses,and the cell death rate decreased by 69.6%under the coexistence of aldehyde and oxidation stress.Compared with GP-CTT,the specific growth rate,glucose consumption rate and ethanol production rate of GP-AC increased by 64.2%,60.1%,and 58.9%,respectively.At the same time,the catalase activity of the recombinant strain increased by 40.2%compared with the single genetic part.In conclusion,this study systematically improved the stress tolerance of S.cerevisiae by rationally designing the genetic circuit of redox-sensitive genetic parts and strengthening the intracellular vital antioxidant enzymes and aldehyde degradation pathways.It provides new insights for rationally designing and constructing feedback genet

关 键 词：氧化还原电位 基因元件 酿酒酵母 胁迫耐受性 木质纤维素 燃料乙醇 

分 类 号：Q939.9[生物学—微生物学]