缺氧微环境对U2OS骨肉瘤细胞化疗耐药性的影响  

Effect of Hypoxic Microenvironment on Chemotherapy Resistance in U2OS Osteosarcoma Cells

作　　者：黄成校[1] 余化龙[1] 高超[1] 冯浩 武伦 HUANG Cheng-xiao;YU Hua-long;GAO Chao;FENG Hao;WU Lun(Sinopharm Dongfeng General Hospital,Hubei University of Medicine,Shiyan,Hubei 442008,China)

机构地区：[1]湖北医药学院附属国药东风总医院,湖北十堰442008

出　　处：《湖北医药学院学报》2023年第6期590-593,共4页Journal of Hubei University of Medicine

基　　金：十堰市科技局引导性项目(21Y88)。

摘　　要：目的:检测抗肿瘤药物在低氧浓度下骨肉瘤U2OS细胞中发挥的作用,从而探讨缺氧微环境对骨肉瘤U2OS细胞化疗耐药性的影响。方法:通过体外实验培养骨肉瘤U2OS细胞,用不同浓度低氧诱导剂CoCl_(2)处理细胞24 h,CCK-8检测各组细胞增殖情况,确立CoCl_(2)干预的最佳浓度;加入不同浓度顺铂后用CCK-8检测各组骨肉瘤细胞的增殖情况,流式细胞术检测各组细胞凋亡比例情况。结果:当骨肉瘤U2OS细胞处于缺氧环境时,其增殖能力增强,与对照组相比有明显差异性(P<0.05);CCK-8结果显示,当处于缺氧环境时,抗肿瘤药物能够抑制骨肉瘤U2OS细胞的增殖并呈剂量依赖性;流式细胞术检测结果显示,经顺铂处理后的骨肉瘤U2OS细胞凋亡率与药物浓度呈正相关(P<0.05)。结论:低氧环境下的骨肉瘤U2OS细胞具有更强的增殖能力,并拮抗抗肿瘤药物的增殖抑制和促进凋亡,因此化疗耐药性增强。Objective To detect the effect of anti-tumor drugs in osteosarcoma U2OS cells under low oxygen concentration,so as to explore the impact of hypoxic microenvironment on the chemotherapy resistance of osteosarcoma U2OS cells.Meth⁃ods U2OS osteosarcoma cells were cultured through in vitro experiments,and cells were treated with different concentra⁃tions of low oxygen inducer CoCl_(2) for 24 hours.The proliferation of cells in each group was detected by CCK-8,and the op⁃timal concentration of CoCl_(2) intervention was established.After adding different concentrations of cisplatin,the proliferation of osteosarcoma cells in each group was detected using CCK-8,and the proportion of cell apoptosis in each group was de⁃tected by flow cytometry.Results When osteosarcoma U2OS cells were in hypoxic environment,their proliferation ability was enhanced,and there was a significant difference compared with the control group(P<0.05).CCK-8 results showed that when the cells were in hypoxic environment,antitumor drugs could inhibit the proliferation of osteosarcoma U2OS cells in a dose-dependent manner.Flow cytometry results showed that the apoptosis rate of osteosarcoma U2OS cells after cispla⁃tin treatment was positively correlated with the drug concentration(P<0.05).Conclusion Osteosarcoma U2OS cells have stronger proliferative ability under hypoxic conditions and antagonize antitumor drug-mediated the proliferation inhibition and apoptotic promotion,thereby enhancing chemotherapy resistance.

关 键 词：骨肉瘤 缺氧微环境 抗肿瘤药物 化疗耐药性 

分 类 号：R738.1[医药卫生—肿瘤]