hsa_circ_100395对胃癌细胞HGC-27、MGC-803增殖、侵袭和迁移的影响  

Effects of hsa_circ_100395 on proliferation, invasion and migration of HGC-27 andMGC-803 gastric cancer cells

作　　者：毛星荐 高其亮 夏冬静 师权 MAO Xingjian;GAO Qiliang;XIA Dongjing;SHI Quan(Department of Medical Laboratory,the People's Hospital of Dujiangyan,Chengdu 611830,Sichuan,China;Department of Gastroenterology,the People's Hospital of Dujiangyan,Chengdu 611830,Sichuan,China)

机构地区：[1]都江堰市人民医院医学检验科,四川成都611830 [2]都江堰市人民医院消化内科,四川成都611830

出　　处：《中南医学科学杂志》2023年第6期858-862,共5页Medical Science Journal of Central South China

基　　金：四川省科技计划项目(2019YFS0082)。

摘　　要：目的探究hsa_circ_100395对胃癌细胞HGC-27、MGC-803增殖、侵袭和迁移的影响。方法收集72例胃癌患者胃癌组织及其癌旁组织样本,采用qRT-PCR检测hsa_circ_100395在胃癌组织中的表达情况,并分析其与临床病理特征的关系。将胃癌细胞系HGC-27和MGC-803分成过表达组(转染hsa_circ_100395过表达质粒)、沉默组(转染hsa_circ_100395沉默质粒)和对照组(转染空质粒)。采用CCK-8法、划痕愈合实验、Transwell法、裸鼠成瘤实验检测胃癌细胞增殖、迁移和侵袭能力。结果hsa_circ_100395在胃癌组织中表达明显下调。hsa_circ_100395低表达与高表达在组织分化程度、浸润深度及TNM分期上差异有显著性(P<0.05)。与对照组比较,hsa_circ_100395过表达显著抑制HGC-27和MGC-803细胞增殖、迁移、侵袭及体内肿瘤的生长;hsa_circ_100395沉默显著促进了HGC-27和MGC-803细胞增殖、迁移、侵袭及体内肿瘤的生长。结论hsa_circ_100395在胃癌组织中低表达,且过表达hsa_circ_100395可抑制胃癌细胞HGC-27、MGC-803增殖、侵袭和迁移。Aim To explore the effects of hsa_circ_100395 on proliferation,invasion and migration of gastric cancer cells.Methods The gastric cancer tissues and adjacent tissue samples were collected from 72 patients with gastric cancer,qRT-PCR was used to detect the expression of hsa_circ_100395 in gastric cancer tissues,and the relationship with clinicopathological characteristics was analyzed.The gastric cancer cell lines HGC-27 and MGC-803 were divided into overexpression group(transfected with hsa_circ_100395 overexpression plasmid),silence group(transfected with hsa_circ_100395 silent plasmid)and control group(transfected with empty plasmid).CCK-8 method,scratch healing test,Transwell method,and nude mouse tumor formation test were used to detect the proliferation,migration and invasion ability of gastric cancer cells.Results The expression of hsa_circ_100395 was significantly down-regulated in gastric cancer.There were significant differences in tissue differentiation,infiltration depth,and TNM staging between low expression and high expression of hsa_circ_100395(P<0.05).Compared with the control group,the overexpression of hsa_circ_100395 significantly inhibited the proliferation,migration,invasion of HGC-27 and MGC-803 cells and tumor growth,whereas hsa_circ_100395 silence significantly promoted the proliferation,migration,invasion of HGC-27 and MGC-803 cells and tumor growth.Conclusion Hsa_circ_100395 is expressed at low level in gastric cancer tissues,and overexpression of hsa_circ_100395 can inhibit the proliferation,invasion and migration of gastric cancer cells.

关 键 词：胃癌 hsa_circ_100395 增殖 侵袭 迁移 

分 类 号：R735.2[医药卫生—肿瘤]