Fundamentals and application in phytoremediation of an efficient arsenate reducing bacterium Pseudomonas putida ARS1  

作　　者：Ze-Wen Wang Guang Yang Jian Chen Yaoyu Zhou Avelino Nunez Delgado Hui-Ling Cui Gui-Lan Duan Barry P.Rosen Yong-Guan Zhu 

机构地区：[1]Henan Institute of Advanced Technology,Zhengzhou University,Zhengzhou 450052,China [2]State Key Lab of Urban and Regional Ecology,Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085,China [3]Department of Cellular Biology and Pharmacology,Florida International University,Herbert Wertheim College of Medicine,Miami,FL,33199,USA [4]University of Chinese Academy of Sciences,Beijing 100049,China [5]Key Laboratory of Urban Environment and Health,Institute of Urban Environment,Chinese Academy of Sciences,Xiamen 361021,China [6]College of Resources and Environment,Hunan Agricultural University,Changsha 410128,China [7]Department of Soil Science and Agricultura Chemistry,Engineering Polytechnic School,University of Santiago de Compostela,Campus Univ.s/n,27002,Lugo,Spain

出　　处：《Journal of Environmental Sciences》2024年第3期237-244,共8页环境科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (Nos.41991332,41977323 and 42090063);the National Institutes of Health (No.R35 GM136211)。

摘　　要：Arsenic is a ubiquitous environmental pollutant.Microbe-mediated arsenic biotransformations significantly infuence arsenic mobility and toxicity.Arsenic transformations by soil and aquatic organisms have been well documented,while little is known regarding effects due to endophytic bacteria.An endophyte Pseudomonas putida ARS1 was isolated from rice grown in arsenic contaminated soil.P.putida ARS1 shows high tolerance to arsenite(As(Ⅲ))and arsenate(As(V)),and exhibits efficient As(V)reduction and As(Ⅲ)effux activities.When exposed to 0.6 mg/L As(V),As(V)in the medium was completely converted to As(Ⅲ)by P.putida ARS1 within 4 hr.Genome sequencing showed that P.putida ARS1 has two chromosomal arsenic resistance gene clusters(arsRCBH)that contribute to efficient As(V)reduction and As(Ⅲ)effux,and result in high resistance to arsenicals.Wolffia globosa is a strong arsenic accumulator with high potential for arsenic phytoremediation,which takes up As(Ⅲ)more efficiently than As(V).Co-culture of P.putida ARS1 and W.globosa enhanced arsenic accumulation in W.globosa by 69%,and resulted in 91%removal of arsenic(at initial concentration of 0.6 mg/L As(V))from water within 3 days.This study provides a promising strategy for in situ arsenic phytoremediation through the cooperation of plant and endophytic bacterium.

关 键 词：ARSENIC Pseudomonas putida Arsenate reduction ENDOPHYTE Wolffia globosa PHYTOREMEDIATION 

分 类 号：X17[环境科学与工程—环境科学]