Nonlinear proinflammatory effect of short-term PM_(2.5)exposure:A potential role of lipopolysaccharide  

作　　者：Fangxia Shen Mutong Niu Haoxuan Chen Ting Zhang Jing Li Haijie Tong Yan Wu 

机构地区：[1]School of Space and Environment,Beihang University,Beijing 100191,China [2]College of Environmental Sciences and Engineering,Peking University,Beijing 100871,China [3]Institute of Surface Science,Helmholtz-Zentrum Hereon,Geesthacht 21502,Germany [4]School of Environmental Science and Engineering,Shandong University,Jinan 250100,China

出　　处：《Journal of Environmental Sciences》2024年第2期292-300,共9页环境科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (Nos.42075117 and 41807306)。

摘　　要：The association between PM_(2.5)(particulate matter≤2.5μm)short-term exposure and its health effect is non-linear from the epidemiological studies.And this nonlinearity is suggested to be related with the PM_(2.5)heterogeneity,however,the underlying biological mechanism is still unclear.Here,a total of 38 PM_(2.5)filters were collected continuously for three weeks in winter Beijing,with the ambient PM_(2.5)varying between 10 and 270μg/m^(3).Human monocytes-derived macrophages(THP-1)were treated with PM_(2.5)water-soluble elutes at10μg/mL to investigate the PM_(2.5)short-term exposure effect from a proinflammatory perspective.The proinflammatory cytokine tumor necrosis factor(TNF)induced by the PM_(2.5)elutes at equal concentrations were unequal,showing the heterogeneity of PM_(2.5)proinflammatory potentials.Of the various chemical and biological components,lipopolysaccharide(LPS)showed a strong positive association with the TNF heterogeneity.However,some outliers were observed among the TNF-LPS association.Specifically,for PM_(2.5)from relatively clean air episodes,the higher LPS amount corresponded to relatively low TNF levels.And this phenomenon was also observed in the promotion tests by treating macrophages with PM_(2.5)elutes dosed with additional trace LPS.Gene expression analysis indicated the involvement of oxidative-stress related genes in the LPS signaling pathway.Therefore,a potential oxidative-stress-mediated suppression on the PM_(2.5)-borne LPS proinflammatory effect was proposed to be accounted for the outliers.Overall,the results showed the differential role of LPS in the heterogeneity of PM_(2.5)proinflammatory effects from a component-based perspective.Future experimental studies are needed to elucidate the signaling pathway of LPS attached on PM_(2.5)from different air quality episodes.

关 键 词：Fine PM PM_(2.5)pro-inflammatory effects Aerosol health effect ENDOTOXIN Oxidative stress 

分 类 号：X513[环境科学与工程—环境工程] R363[医药卫生—病理学]