Transcriptomics and physiological analyses reveal that sulfur alleviates mercury toxicity in rice(Oryza sativa L.)  

作　　者：Yingmei Huang Jicai Yi Xiaomin Li Fangbai Li 

机构地区：[1]National-Regional Joint Engineering Research Center for Soil Pollution Control and Remediation in South China,Guangdong Key Laboratory of Integrated Agro-Environmental Pollution Control and Management,Institute of Eco-Environmental and Soil Sciences,Guangdong Academy of Sciences,Guangzhou 510650,China [2]Guangzhou Institute of Geochemistry,Chinese Academy of Sciences,Guangzhou 510640,China [3]University of Chinese Academy of Sciences,Beijing 100049,China [4]College of Life Sciences,South China Agricultural University,Guangzhou 510642,China [5]SCNU Environmental Research Institute,Guangdong Provincial Key Laboratory of Chemical Pollution and Environmental Safety&MOE Key Laboratory of Theoretical Chemistry of Environment,South China Normal University,Guangzhou 510006,China

出　　处：《Journal of Environmental Sciences》2024年第1期10-25,共16页环境科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (Nos.42030702 and 41877143);the Guangdong Key Research and Development Project (No.2019B110207002);the Local Innovative and Research Teams Project of the Guangdong Pearl River Talents Program (No.2017BT01Z176)。

摘　　要：Mercury(Hg)is one of the most dangerous contaminants and has sparked global concern since it poses a health risk to humans when consumed through rice.Sulfur(S)is a crucial component for plant growth,and S may reduce Hg accumulation in rice grains.However,the detailed effects of S and the mechanisms underlying S-mediated responses in Hg-stressed rice plants remain unclear.Currently,to investigate the effects of S addition on rice growth,Hg accumulation,physiological indexes,and gene expression profiles,rice seedlings were hydroponically treated with Hg(20μmol/L Hg Cl_(2))and Hg plus elemental sulfur(100 mg/L).S application significantly reduced Hg accumulation in Hg-stressed rice roots and alleviated the inhibitory effects of Hg on rice growth.S addition significantly reduced Hg-induced reactive oxygen species generation,membrane lipid peroxidation levels,and activities of antioxidant enzymes while increasing glutathione content in leaves.Transcriptomic analysis of roots identified 3,411,2,730,and 581 differentially expressed genes in the control(CK)vs.Hg,CK vs.Hg+S,and Hg vs.Hg+S datasets,respectively.The pathway of S-mediated biological metabolism fell into six groups:biosynthesis and metabolism,expression regulation,transport,stimulus response,oxidation reduction,and cell wall biogenesis.The majority of biological process-related genes were upregulated under Hg stress compared with CK treatment,but downregulated in the Hg+S treatment.The results provide transcriptomic and physiological evidence that S may be critical for plant Hg stress resistance and will help to develop strategies for reduction or phytoremediation of Hg contamination.

关 键 词：SULFUR MERCURY TRANSCRIPTOMICS DETOXIFICATION 

分 类 号：X173[环境科学与工程—环境科学] S511[农业科学—作物学]