Structural effects of sulfonamides on the proliferation dynamics of sulfonamide resistance genes in the sequencing batch reactors and the mechanism  

作　　者：Ligui Wu Mingyu Wang Lingling Rong Wenbiao Wang Linwei Chen Qiaofeng Wu Haoyu Sun Xiangfeng Huang Xiaoming Zou 

机构地区：[1]College of Environmental Science and Engineering,Tongji University,Shanghai 200092,China [2]School of Life Science,Jinggangshan University,Ji’an 343009,China [3]Key Laboratory of Organic Compound Pollution Control Engineering(MOE),School of Environmental and Chemical Engineering,Shanghai University,Shanghai 200444,China [4]Fuzhou Urban and Rural Construction Group Co.Ltd.,Fuzhou 350007,China

出　　处：《Journal of Environmental Sciences》2024年第1期161-173,共13页环境科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (Nos.32160303 and 31760165);the Education Department of Jiangxi Province (No.GJJ211001);the Scientific and Technological Project of Ji’an City (No.20211–025333);the Key Project of Natural Science Foundation of Jiangxi Province (2022)。

摘　　要：Antibiotic resistance genes(ARGs)can be easily promoted by antibiotics,however,the structural effects of antibiotics on the proliferation of ARGs dynamic and the associated mechanisms remain obscure in,especially,activated sludge sequencing batch reactors.In the present study,the effects of 9 sulfonamides(SAs)with different structures on the proliferation dynamic of sulfonamide resistance genes(Suls)in the activated sludge sequencing batch reactors and the corresponding mechanisms were determined(30 days),and the results showed that the largest proliferation value(ΔA^(R))of Suls dynamic for SAs(sulfachloropyridazine)was approximately 2.9 times than that of the smallest one(sulfadiazine).The proliferation of Suls was significantly related to the structural features(minHBint6,SssNH,SHBd and SpMax2_Bhm)that represent the biological activity of SAs.To interpret the phenomenon,a mechanistic model was developed and the results indicated that the biodegradation of SAs(T_(1/2))rather than conjugative transfer frequency or mutation frequency tends to be the key process for affecting Suls proliferation.T_(1/2)was proved to be dependent on the interactions between SAs and receptors(E_(binding)),the cleavage mode(bond dissociation energy),and the site of nucleophilic assault.Besides,the metagenomic analysis showed that SAs posed significant effect on antibiotic resistome and Tnp31 played a vital role in the proliferation of Suls.Overall,our findings provide important insight into a theoretical basis for understanding the structural effects of SAs on the proliferation of ARGs in SBR systems.

关 键 词：Activated sludge SULFONAMIDES Suls PROLIFERATION MECHANISM 

分 类 号：X703[环境科学与工程—环境工程]