Multi-endpoint assays reveal more severe toxicity induced by chloraminated effluent organic matter than chloraminated natural organic matter  

作　　者：Hai-Yan Wang De-Xiu Wu Ye Du Xiao-Tong Lv Qian-Yuan Wu 

机构地区：[1]State Key Laboratory of Environmental Criteria and Risk Assessment,Chinese Research Academy of Environmental Sciences,Beijing 100012,China [2]Environmental Protection Key Laboratory of Microorganism Application and Risk Control,Guangdong Provincial Engineering Research Center for Urban Water Recycling and Environmental Safety,Tsinghua Shenzhen International Graduate School,Tsinghua University,Shenzhen 518055,China [3]College of Architecture and Environment,Sichuan University,Chengdu 610000,China

出　　处：《Journal of Environmental Sciences》2024年第1期310-317,共8页环境科学学报（英文版）

基　　金：supported by the National Key R&D Program of China (No.2020YFC1806302);National Natural Science Foundation of China (No.52022049/51978370);Guangdong Basic and Applied Basic Research Foundation (No.2020A1515110106)。

摘　　要：Disinfection by chloramination produces toxic byproducts and the difference in toxicity of reclaimed and drinking water treated by chloramination remains unclear.This study investigated cytotoxic effects at the same concentrations of dissolved organic matter and showed that chloraminated effluent organic matter(EfOM)induced 1.7 times higher cytotoxicity than chloraminated natural organic matter(NOM)applied to simulate drinking water.Chloraminated EfOM induced more reactive nitrogen species than chloraminated NOM,and chloraminated EfOM and NOM induced similar and higher levels of reactive oxygen species than the negative control,respectively.Consequently,intracellular macromolecule damage indicated by DNA/RNA damage marker 8–hydroxy-(deoxy)guanosine and the intracellular protein carbonyl concentration induced by chloraminated EfOM was higher and slightly more than that induced by chloraminated NOM,respectively.These data were consistent with the effects on cell physiological processes.Cell cycle arrest mainly occurred in G2 phase by chloraminated EfOM and NOM.Early apoptotic cells,which could return to normal,increased upon exposure to high concentrations of chloraminated EfOM and NOM.Moreover,necrotic cells were significantly increased from 0.5%to 2.5%when the concentration increased from 20-to 60-fold chloraminated EfOM,but were not obviously changed by chloraminated NOM.These results indicated that the comprehensive intracellular changes induced by toxic substances in chloraminated EfOM were more irreversible and induced more cell death than chloraminated NOM.

关 键 词：CYTOTOXICITY Intracellular active nitrogen/oxygen Intracellular oxidative damage Cell necrosis Reclaimed water 

分 类 号：X703[环境科学与工程—环境工程]