浒苔抑菌成分的提取工艺优化及其活性研究  

Optimization of Extraction Process and Antibacterial Activity Evaluation on the Antibacterial Active Components from Enteromorpha clathrata

作　　者：张中堂 陈天乐 柏亚萱 盘赛昆 ZHANG Zhongtang;CHEN Tianle;BAI Yaxuan;PAN Saikun(School of Ocean Food and Biological Engineering,Jiangsu Ocean University,Lianyungang 222005,China;Jiangsu Key Laboratory of Marine Biotechnology,Jiangsu Collaborative Innovation Center of Marine Biological IndustryTechnology,Jiangsu Ocean University,Lianyungang 222005,China)

机构地区：[1]江苏海洋大学海洋食品与生物工程学院,江苏连云港222005 [2]江苏海洋大学江苏省海洋生物技术重点实验室、江苏省海洋生物产业技术协同创新中心,江苏连云港222005

出　　处：《林产化学与工业》2023年第6期123-129,共7页Chemistry and Industry of Forest Products

基　　金：江苏省高等学校自然科学研究重大项目(21KJA240004)。

摘　　要：在对条浒苔水提取物和乙醇提取物进行抑菌实验的基础上,通过单因素试验和响应面分析法优化乙醇提取工艺,并对乙醇提取物中的主要成分进行常规定性分析,进一步研究了不同溶剂萃取物的抑菌效果,最后采用GC-MS对乙酸乙酯萃取物进行分析。研究结果表明:与水提取物相比,条浒苔乙醇提取物的抑菌效果更好,对金黄色葡萄球菌有较好的抑制效果。响应面分析法优化得到的最佳工艺条件为:乙醇体积分数92%、液料比30∶1、提取温度83℃、提取时间4 h,此条件下,乙醇提取物对金黄色葡萄球菌的抑菌圈直径为(1.152±0.015)cm。常规定性分析发现条浒苔乙醇提取物中含有有机酸类、甾醇及萜类等成分,不含生物碱、蒽醌、酚类、皂苷、黄酮类等成分。乙酸乙酯萃取物的抑菌效果最好(抑菌圈直径2.82 cm),二氯甲烷和石油醚萃取物次之(抑菌圈直径分别为1.68和1.06 cm),正丁醇和水萃取物没有抑菌效果。GC-MS分析发现乙酸乙酯萃取物中含有12种挥发性成分,主要为酸类化合物、芳香类化合物。On the basis of conducting antibacterial experiments on the water extract and ethanol extract of Enteromorpha clathrata,the ethanol extraction process was optimized through single factor experiments and response surface analysis.The main components in the ethanol extract were conventionally qualitatively analyzed,and the antibacterial effects of different solvent extracts were further studied.Finally,the ethyl acetate extract was analyzed using GC-MS.The results showed that the ethanol extract of E.clathrata had better antibacterial effect and better inhibitory effect on Staphylococcus aureus than that of the water extract.The optimal process conditions obtained by response surface analysis were as follows:ethanol volume fraction of 92%,liquid material ratio of 30∶1,extraction temperature of 83℃,extraction time of 4 h.Under this condition,the diameter of the inhibitory zone of ethanol extract against S.aureus was(1.152±0.015)cm.Conventional qualitative analysis found that the ethanol extract of E.clathrata contains organic acids,sterols,and terpenoids,but did not contain alkaloids,anthraquinone,phenols,saponins,flavonoids and other components.The ethyl acetate extract had the best bacteriostatic effect(the inhibition zone diameter was 2.82 cm),followed by dichloromethane and petroleum ether extracts(the inhibition diameters were 1.68 and 1.06 cm,respectively),and n-butanol and water extracts had no bacteriostatic effect.GC-MS analysis showed that the ethyl acetate extract contained 12 volatile components,mainly acid compounds and aromatic compounds.

关 键 词：条浒苔 活性提取物 优化工艺 抑菌效果 

分 类 号：TQ35[化学工程]