RUNX1在AngⅡ诱导心肌肥厚中的作用及机制  

Role and mechanism of RUNX1 in Ang Ⅱ-induced cardiac hypertrophy

作　　者：丁立群 王玉玖[2] 杨丽娟[2] 沈嘉祺 王傲 邹明锐 刘宝辉[2] DING Liqun;WANG Yujiu;YANG Lijuan;SHEN Jiaqi;WANG Ao;ZOU Mingrui;LIU Baohui(Binzhou Medical University,Yantai 264003,Shandong,P.R.China;Binzhou Medical University Hospital,Binzhou 256603,Shandong,P.R.China)

机构地区：[1]滨州医学院,山东烟台264003 [2]滨州医学院附属医院,山东滨州256603

出　　处：《滨州医学院学报》2023年第6期401-405,共5页Journal of Binzhou Medical University

基　　金：山东省自然科学基金(ZR2020QH017);山东省医药卫生科技发展计划(2019WS327)。

摘　　要：目的探讨Runt相关转录因子1(RUNX1)对血管紧张素Ⅱ(AngⅡ)诱导的心肌肥厚的作用及机制。方法将H9C2心肌细胞分为Control组、L-AngⅡ组、M-AngⅡ组、H-AngⅡ组,验证AngⅡ诱导心肌肥厚模型并检测不同AngⅡ浓度下RUNX1表达情况。将H9C2心肌细胞分为慢病毒转染无义序列(NS)组、AngⅡ+NS组、shRUNX1组、AngⅡ+shRUNX1组检测RUNX1对心肌肥厚的影响。采用Western blot法检测各组细胞RUNX1、SERCA2a表达量的变化,采用qRT-PCR法检测各组细胞心肌肥厚相关指标,采用免疫荧光法检测心肌细胞面积。结果在H9C2中加入AngⅡ48 h后,与对照组相比,AngⅡ处理后细胞中RUNX1蛋白表达量明显上调,并呈剂量依赖关系。转染shRUNX172 h后进行AngⅡ处理,与AngⅡ+NS组相比,AngⅡ+shRUNX1组BNP mRNA、RUNX1蛋白表达量明显降低,心肌细胞面积显著减小;而SERCA2a蛋白表达量明显上调。结论RUNX1参与AngⅡ诱导的心肌肥厚的病理过程,其作用机制可能与抑制SERCA2a相关。Objective To investigate the effect of Runt-related transcription factor 1(RUNX1)on cardiac hypertrophy induced by angiotensin Ⅱ(Ang Ⅱ)and its mechanism.Methods H9C2 cardiomyocytes were divided into the control group,the L-Ang Ⅱ group,the M-Ang Ⅱ group and the H-Ang Ⅱ group.Ang Ⅱ-induced cardiac hypertrophy model was established and the expression of RUNX1 was detected under different concentrations of Ang Ⅱ.H9C2 cardiomyocytes were divided into the NS group,the Ang Ⅱ+NS group,the shRUNX1 group and the Ang Ⅱ+shRUNX1 group to detect the effect of RUNX1 on cardiomyocyte hypertrophy.Western blot was used to detect the expression of RUNX1 and SERCA2a.qRT-PCR was used to detect the related indexes of cardiac hypertrophy,and immunofluorescence was used to detect the myocardial cell area.Results Compared with that in the control group,the expression of RUNX1 protein in H9C2 cells treated with Ang Ⅱ for 48 h was up-regulated in a dose-dependent manner.Compared with that in the Ang Ⅱ+NS group,the expression of BNP mRNA and RUNX1 protein in the Ang Ⅱ+shRUNX1 group decreased significantly,and the area of cardiomyocytes decreased significantly.The expression of SERCA2a protein was up-regulated.Conclusion RUNX1 is involved in the pathogenesis of Ang Ⅱ-induced cardiac hypertrophy,and its mechanism may be related to the inhibition of SERCA2a.

关 键 词：心肌肥厚 血管紧张素Ⅱ Runt相关转录因子1 SERCA2A 

分 类 号：R542.2[医药卫生—心血管疾病]