NOS3单核苷酸多态性检测在妊娠高血压患者中的表达及临床指导价值研究  

Study on expression and clinical guiding value of NOS3 single nucleotide polymorphism detection in pregnant patients with hypertension

作　　者：曾张琴[1] 韦东[1] 巢微 Zeng Zhangqin;Wei Dong;Chao Wei(Liuzhou Worker's Hospital&the Fourth Affiliated Hospital of Guangxi Medical University,Liuzhou,Guangxi 545005,China)

机构地区：[1]广西医科大学第四附属医院柳州工人医院,广西柳州545005

出　　处：《齐齐哈尔医学院学报》2023年第19期1845-1848,共4页Journal of Qiqihar Medical University

基　　金：广西壮族自治区卫生厅自筹经费科研课题(2014681)。

摘　　要：目的研究内皮型一氧化氮合酶(Nitric oxide synthase 3,NOS3)单核苷酸多态性检测在妊娠高血压患者中的表达及临床指导价值。方法选择2016年3月—2018年1月本院收治的妊娠高血压患者(妊娠高血压组,74例)和正常妊娠孕妇(正常妊娠组,80名)作为研究对象,采集孕妇外周血,提取DNA,PCR-RFLP技术分析NOS3基因Glu298Asp位点的基因型。分析妊娠高血压患者NOS3基因Glu298Asp位点基因型、等位基因与妊娠高血压严重程度关系。分析妊娠高血压患者并发症、妊娠不良结局患者与基因型频率关系。结果正常妊娠组和妊娠高血压组孕妇NOS3基因Glu298Asp位点TT、GT、GG基因型频率比较,差异存在统计学意义(P<0.05)。正常妊娠组和妊娠高血压组孕妇NOS3基因Glu298Asp位点等位基因G和T频率比较,差异存在统计学意义(P<0.05)。比较轻度、中度和重度妊娠高血压患者NOS3基因Glu298Asp位点基因型频率,差异无统计学意义(P>0.05)。轻度、中度、重度妊娠高血压患者NOS3基因Glu298Asp位点等位基因T频率逐渐升高。基因型为GT的妊娠高血压患者并发胎盘早剥、左心力衰竭、HELLP综合征、DIC、急性肾功能衰竭总发生率明显高于基因型TT和基因型GG患者(P<0.05)。基因型为GT的妊娠高血压患者新生儿窒息、剖宫产、胎儿宫内窘迫、围生儿死亡妊娠不良结局总发生率明显高于基因型TT和基因型GG患者(P<0.05)。结论NOS3基因Glu298Asp位点基因型、等位基因频率与妊娠高血压有关,等位基因T频率越高,妊娠高血压严重程度增加,基因型为GT的妊娠高血压患者并发症和妊娠不良结局的总发生率增加。Objective To investigate the expression of endothelial nitric oxide synthase 3(NOS3)single nucleotide polymorphism in pregnant patients with hypertension and its clinical guiding value.Methods 74 pregnant patients with gestational hypertension(gestational hypertension group)and 80 normal pregnant women(normal pregnancy group)who were admitted to our hospital during January 2016 and January 2018,were selected as the study subjects.Peripheral blood of pregnant women was collected,DNA was extracted,and the Glu298Asp site genotype of NOS3 was analyzed by PCR-RFLP.The relationship between the Glu298Asp locus genotype and allele of NOS3 gene and the severity of gestational hypertension in patients with gestational hypertension was analyzed.The relationship between genotype frequency and complications of pregnancy-induced hypertension and adverse pregnancy outcome were analyzed.Results There were statistically significant differences in the frequency of TT,GT and GG genotypes of Glu298Asp locus of NOS3 gene between normal pregnancy group and gestational hypertension group(P<0.05).The difference in frequency of allele G and T of Glu298Asp locus of NOS3 gene in normal pregnancy group compared with gestational hypertension group was statistically significant(P<0.05).There was no significant difference in the Glu298Asp locus genotype frequency of NOS3 gene among patients with mild,moderate and severe gestational hypertension(P>0.05).The T frequency of Glu298Asp locus allele in NOS3 gene increased gradually in patients with mild,moderate and severe gestational hypertension.The total incidence of placental abruption,left heart failure,HELLP syndrome,DIC and acute renal failure in pregnant hypertensive patients with genotype GT was significantly higher than those with genotype TT and genotype GG(P<0.05).The total incidence of neonatal asphyxia,cesarean section,fetal distress and perinatal death in hypertensive patients with GT was significantly higher than those with TT and GG genotype(P<0.05).Conclusions The genotype,allele f

关 键 词：NOS3单核苷酸多态性 Glu298Asp位点 妊娠高血压 基因型 等位基因 

分 类 号：R714.2[医药卫生—妇产科学]