CircPARD3调控miR-326/CXCL3轴对口腔鳞状细胞癌细胞迁移、侵袭及上皮间质转化的影响  

CircPARD3 regulates migration,invasion,and epithelial mesenchymal transition of oral squamous cell carcinoma cell by regulating the miR-326/CXCL3 axis

作　　者：尹江[1] 唐平[1] 张慧[1] 杨森[1] YIN Jiang;TANG Ping;ZHANG Hui(Oral Cavity Center,Suining Central Hospital,Sichuan,Suining 629000,China)

机构地区：[1]四川省遂宁市中心医院口腔医学中心,629000

出　　处：《河北医药》2023年第24期3697-3702,共6页Hebei Medical Journal

基　　金：四川省科技计划项目(编号:2018PZ0113)。

摘　　要：目的探究环状RNA PARD3(circPARD3)靶向微小RNA-326(miR-326)/趋化因子配体3(CXCL3)轴对口腔鳞状细胞癌(OSCC)细胞迁移、侵袭及上皮间质转化(EMT)的影响。方法qRT-PCR法分析OSCC组织及细胞中circPARD3和miR-326表达水平。双荧光素酶实验验证circPARD3和miR-326、CXCL3和miR-326的靶向关系。在OSCC细胞CAL-27中转染si-NC、si-circPARD3、si-circPARD3+anti-NC、si-circPARD3+anti-miR-326、miR-NC、miR-326 mimics,将未转染的CAL-27细胞设为对照组。平板克隆实验检测细胞增殖;细胞划痕实验检测细胞迁移;Transwell实验检测细胞侵袭能力。Western blot法检测上皮细胞钙粘蛋白(E-cadherin)、神经性钙黏附蛋白(N-cadherin)和波形蛋白(Vimentin)的蛋白表达。小鼠移植瘤实验验证circPARD3对OSCC肿瘤生长及miR-326/CXCL3的影响。结果在OSCC组织与细胞中,circPARD3表达上调,miR-326表达下调,抑制circPARD3表达可以显著抑制OSCC细胞增殖、迁移、侵袭及EMT。抑制miR-326可以逆转抑制circPARD3表达对OSCC细胞增殖、迁移、侵袭及EMT的抑制作用。小鼠移植瘤实验结果显示,抑制circPARD3表达,移植瘤体积及质量降低,miR-326表达水平升高,CXCL3表达水平降低。结论circPARD3在OSCC组织与细胞中表达上调,抑制circPARD3表达,上调miR-326表达,进而抑制CXCL3表达,抑制OSCC细胞迁移、侵袭及EMT。Objective To investigate the regulatory effects of cyclic RNA PARD3(circPARD3)on migration,invasion and epithelial mesenchymal transformation(EMT)of oral squamous cell carcinoma(OSCC)cells by targeting the microRNA-326(miR-326)/CXC chemokine ligand 3(CXCL3)axis.Methods Expression levels of circPARD3 and miR-326 in OSCC tissues and cells were detected by quantitative real-time polymerase chain reaction(qRT-PCR).The target relationship between circPARD3 and miR-326,and that between miR-326 and CXCL3 was verified by the dual-luciferase reporter assay.The OSCC cell line CAL-27 was transfected with si-NC,si-circPARD3,si-circPARD3+anti-NC,si-circPARD3+anti-miR-326,miR-NC,and miR-326 mimics respectively,and CAL-27 cells treated with blank control were set as the control group.Cell proliferation,migration,and invasion were detected by colony formation assay,wound healing assay and Transwell assay,respectively.Western blot was applied to detect the protein expressions of E-cadherin,N-cadherin and Vimentin in CAL-27 cells.An in vivo OSCC xenograft mouse model was created to verify the effects of circPARD3 on OSCC tumor growth and the involvement of the miR-326/CXCL3 axis.Results In OSCC tissues and cells,circPARD3 was up-regulated,while miR-326 was down-regulated.Knockdown of circPARD3 significantly inhibited OSCC cell proliferation,migration,invasion,and EMT,which were significantly reversed by knockdown of miR-326.The in vivo OSCC xenograft mouse model showed that knockdown of circPARD3 significantly decreased the volume and weight of xenograft tumors,and upregulated miR-326 and downregulated CXCL3.Conclusion CircPARD3 is up-regulated in OSCC tissues and cells,and knockdown of circPARD3 inhibits OSCC cell migration,invasion and EMT by up-regulating miR-326,thereby down-regulating CXCL3 expression.

关 键 词：环状RNA PARD3 微小RNA-326 趋化因子配体3 口腔鳞状细胞癌 迁移 侵袭 上皮间质转化 

分 类 号：R739.8[医药卫生—肿瘤]