外周血炎症因子及肠道菌群特征与活动性溃疡性结肠炎患者病情的相关性分析  

Correlation analysis of inflammatory factors in peripheral blood and characteristics of Gut microbiota with the condition of patients with active Ulcerative colitis

作　　者：屈霄 王靓 陆萍 何斌[1] 孙敏[1] Xiao Qu;Liang Wang;Ping Lu;Bin He;Min Sun(Department of Gastroenterology,Zhangjiagang Hospital Affiliated to Soochow University,Suzhou 215600,China)

机构地区：[1]苏州大学附属张家港医院消化内科,江苏省215600

出　　处：《中华消化病与影像杂志（电子版）》2023年第6期466-470,共5页Chinese Journal of Digestion and Medical Imageology(Electronic Edition)

摘　　要：目的 探讨外周血炎症因子水平及肠道菌群特征与活动性溃疡性结肠炎(UC)患者病情的相关性。方法 采用单中心前瞻性队列研究方法,选择2020年1月至2022年12月在苏州大学附属张家港医院诊治的171例活动性UC患者为研究对象(研究组),根据活动性UC病情分为轻度组(79例)、中度组(47例)、重度组(45例)三个亚组,另选同期院内体检的健康人群100例为对照组。ELISA法测定外周血炎症因子,对比各组外周血内炎症因子与肠道菌群特征情况,分析活动性UC患者病情与外周血内炎症因子及肠道菌群特征的相关性。结果 研究组TNF-α、IL-1 β、IL-6、IL-17水平及大肠杆菌和拟杆菌数量明显高于对照组,双歧杆菌和乳酸杆菌数量明显低于对照组,差异均有统计学意义(P<0.05),两组IL-10和肠球菌水平差异无统计学意义(P>0.05)。多因素logistic分析显示,TNF-α、IL-1β、IL-6、IL-17、大肠杆菌、双歧杆菌、乳酸杆菌、拟杆菌是影响活动性UC患者病情的独立因素(P <0.05),肠球菌不是影响活动性UC患者病情的独立因素(P>0.05)。ROC曲线显示,IL-1β、大肠杆菌、双歧杆菌预测活动性UC病情效果佳,曲线下面积分别为0.869、0.859、0.869,IL-6预测UC病情效果一般,曲线下面积为0.715,TNF-α、乳酸杆菌预测UC病情效果较低(P<0.05)。结论 TNF-α、IL-1 β、IL-6、IL-17、大肠杆菌、双歧杆菌、乳酸杆菌、拟杆菌是影响活动性UC患者病情的独立因素,IL-1 β、大肠杆菌、双歧杆菌预测活动性UC患者病情具有很好价值。ObjectiveTo investigate the correlation between inflammatory factors in peripheral blood and gut microbiota characteristics and the condition of active ulcerative colitis(UC).MethodsA single center prospective cohort method was used.A total of 171 patients with active UC who were diagnosed and treated in Zhangjiagang Hospital Affiliated to Soochow University from January 2020 to December 2022 were selected as the research objects and as the research group.According to the condition of active UC, they were divided into three subgroups: mild group(79 cases), moderate group(47 cases)and severe group(45 case). Another 100 healthy people who were examined in our hospital at the same time were selected as the control group.Inflammatory factors in peripheral blood were detected by enzyme-linked immunosorbent assay(ELISA), the inflammatory factors in peripheral blood and the characteristics of gut microbiota in each group were compared, and the correlation between the condition of active UC patients and the characteristics of inflammatory factors in the peripheral blood and gut microbiota was analyzed.ResultsThe levels of TNF-α, IL-1β, IL-6, IL-17 and the numbers of Escherichia coli and Bacteroides in the research group were significantly higher than those in the control group, while the numbers of Bifidobacteria and Lactobacilli were significantly lower than those in the control group, with statistically significant differences(P<0.05). There was no significant difference in IL-10 level and the number of Enterococcus between the two groups(P>0.05). The results of multivariate logistic analysis suggested that TNF-α, IL-1β, IL-6, IL-17, Escherichia coli, Bifidobacterium, Lactobacillus and Bacteroides were independent factors affecting the condition of patients with active UC(P<0.05). Enterococcus was not an independent factor affecting the condition of patients with active UC(P>0.05). ROC curve results showed that IL-1β, Escherichia coli and Bifidobacteria had a good effect in predicting the condition of patients wit

关 键 词：溃疡性结肠炎 炎症因子 肠道菌群特征 病情 

分 类 号：R574.62[医药卫生—消化系统]