观赏动物犬细小病毒的鉴定及VP2基因的序列分析  

Identification of canine parvovirus and sequence analysis of VP2 gene in ornamental animals

作　　者：庞卓 黄学喆 左珂菁[2] 陈绚姣[2] 罗均[3] 郭霄峰[3] 黄勉[2] PANG Zhuo;HUANG Xuezhe;ZUO Kejing;CHEN xuanjiao;LUO Jun;GUO Xiaofeng;HUANG Mian(Maoming wildlife rescue research center,Maoming Guangdong 525000;Guangzhou Zoo,Guangzhou Guangdong 510070;College of Veterinary Medicine,South China Agricultural University,Guangzhou Guangdong 510642)

机构地区：[1]茂名市野生动物救护研究中心,广东茂名525000 [2]广州动物园,广东广州510070 [3]华南农业大学兽医学院,广东广州510642

出　　处：《广东畜牧兽医科技》2023年第6期46-49,共4页Guangdong Journal of Animal and Veterinary Science

摘　　要：为了对疑似感染犬细小病毒(canine parvovirus;CPV)的海狸鼠和狞猫等观赏动物进行确诊及了解遗传进化情况,将其肛拭子和粪便提取基因组DNA,通过PCR方法鉴定,并对VP2进行基因扩增与测序,和国内外参考株进行遗传进化树分析。结果表明,送检的15份样品均扩增出约为756 bp的特异性条带,与FPV标准株CU-4株序列比对后确定为CPV,阳性率达100%;遗传进化树显示,分离株与北京地区分离得到的CPV-2a、CPV-New2a亚型毒株遗传关系较近,与其他参考株遗传距离相对较远。该研究结果丰富了观赏动物犬细小的研究资料以及该病的遗传和预防提供了参考。In order to confirm the diagnosis and understand the genetic evolution of ornamental animals suspected to be infected with canine parvovirus,such as nutria and caracal cats,genomic DNA was extracted from their anal swabs and feces.The VP2 gene was sequenced by PCR,and the genetic evolution tree was analyzed with Chinese and foreign reference strains.The results showed that 15 of the 15 samples were amplified with a specific band of about 756 bp,which was identified as CPV after comparison with the CU-4 strain of FPV standard,with a positive rate of 100%.The genetic evolution tree showed that the isolates had a close genetic relationship with CPV-2a and CPV-New2a subtypes isolated from Beijing and a relatively long genetic distance from other reference strains.The results of this study enrich the research data of canine parvus in ornamental animals and provide references for the genetics and prevention of the disease.

关 键 词：观赏动物 犬细小病毒 VP2基因 遗传分析 

分 类 号：S855.3[农业科学—临床兽医学]