CD276在泛癌中的免疫表达及预后  

CD276 immune expression and prognosis in pan-cancer

作　　者：张癸乾 王宁[1] 荣耀 唐明政 蔡辉[2,3,4] ZHANG Guiqian;WANG Ning;RONG Yao;TANG Mingzheng;CAI Hui(First Clinical Medical College of Gansu University of Traditional Chinese Medicine,Lanzhou 730000,China;Clinical General Surgery Centre,Gansu Provincial Hospital,Lanzhou 730000,China;NHC Key Laboratory of Diagnosis and Therapy of Gastrointestinal Tumor,Gansu Provincial Hospital,Lanzhou 730000,China;Key Laboratory of Molecular Diagnostics and Precision Medicine for Surgical Oncology in Gansu Province,Gansu Provincial Hospital,Lanzhou 730000,China)

机构地区：[1]甘肃中医药大学第一临床医学院,兰州730000 [2]甘肃省人民医院临床普外中心,兰州730000 [3]国家卫生健康委胃肠肿瘤诊治重点实验室,甘肃省人民医院,兰州730000 [4]甘肃省外科肿瘤分子诊断与精准治疗重点实验室,甘肃省人民医院,兰州730000

出　　处：《中国医科大学学报》2023年第12期1106-1112,1118,共8页Journal of China Medical University

基　　金：2021年中央引导地方科技发展基金(ZYYDDFFZZJ-1);甘肃省人民医院优秀硕博士基金(22GSSYD-19);甘肃省人民医院科技创新平台基金重点项目(21gssya-4)。

摘　　要：目的探讨CD276在泛癌中的表达和对患者预后的影响及其作用机制。方法采用TCGA和GTEx数据库分析CD276在肿瘤组织与正常组织中的差异表达,采用R语言分析CD276对泛癌患者预后、肿瘤微环境、肿瘤突变负荷和微卫星不稳定性的影响,进一步分析肿瘤组织中CD276表达与肿瘤免疫细胞浸润的相关性,采用基因集富集分析(GSEA)CD276的生物学功能,最后采用实时PCR对CD276在肿瘤中的表达进行体外验证。结果肿瘤组织与正常组织间CD276表达存在统计学差异(P<0.05)。CD276影响肿瘤预后并与肿瘤微环境、肿瘤突变负荷、微卫星不稳定性和肿瘤免疫细胞浸润密切相关。结论全面的泛癌分析确定CD276是免疫浸润和癌症预后不良的生物标志物,可将其作为新的免疫调节靶点,用于开发新的免疫治疗靶向药物,为肿瘤治疗提供新思路。Objective To investigate the expression of CD276 in pan-cancer,its effect on patient prognosis,and its mechanism of action.Methods TCGA and GTEx databases were used to study the differential expression of CD276 in tumor and normal tissues;R language was used to analyze the effects of CD276 on the prognosis of patients with pan-cancer,the tumor microenvironment,the tumor mutation burden,and microsatellite instability;the correlation between the expression of CD276 in tumor tissues and the tumor immune cell infiltration was further examined;the biological function of CD276 was analyzed using Gene Set Enrichment Analysis(GSEA);and finally,real-time PCR was used to validate the expression of CD276 in tumors in vitro.Results A statistical difference in CD276 expression was detected between tumor and normal tissues(P<0.05).CD276 affects tumor prognosis and is strongly associated with the tumor microenvironment,tumor mutational burden,microsatellite instability,and tumor immune cell infiltration.Conclusion Comprehensive pan-cancer analysis identified CD276 as a biomarker for immune infiltration and poor cancer prognosis,which can be used as a novel immunomodulatory target for the development of immunotherapeutically-targeted drugs,providing new ideas for tumor therapy.

关 键 词：CD276 肿瘤微环境 免疫 预后 泛癌 

分 类 号：R730[医药卫生—肿瘤]