壳聚糖/BMP-2质粒温敏水凝胶复合体促犬牙槽骨再生的研究  

The promoting effect of chitosan thermosensitive hydrogel complex with chitosan nanoparticles carrying BMP-2 plasmid DNA on alveolar bone regeneration in beagle dogs

作　　者：李慧 吉秋霞[2] Li Hui;Ji Qiuxia(Department of Stomatology,Beijing Friendship Hospital,Capital Medical University,Beijing 100000,China;不详)

机构地区：[1]首都医科大学附属北京友谊医院口腔科,北京100000 [2]青岛大学附属医院口腔科,青岛266071

出　　处：《新医学》2023年第12期872-878,共7页Journal of New Medicine

基　　金：国家自然科学基金青年科学基金(81401526)。

摘　　要：目的 进行壳聚糖/BMP-2质粒温敏水凝胶复合体系CS/CSn(pDNA-BMP2)-GP的构建,对犬牙槽骨再生中此复合体系的作用进行分析。方法 将4只比格犬第2、3前磨牙建立牙周炎模型,实验牙齿随机划分为3组:CS/CSn-GP组、CS/CSn(pDNA-BMP2)-GP组、空白对照组。CS/CSn-GP组注入不含BMP-2的壳聚糖水凝胶复合体(CS/CSn-GP);CS/CSn(pDNA-BMP2)-GP组注入壳聚糖/BMP-2质粒温敏水凝胶复合体CS/CSn(pDNA-BMP2)-GP,空白对照组不注入任何材料。8周后处死,采用Masson染色法观察牙周炎部位牙槽骨再生状况;钙钴法观察骨缺损部位碱性磷酸酶(ALP)表达状况。结果 Masson染色显示CS/CSn-GP组与CS/CSn(pDNA-BMP2)-GP组,均有不同程度的牙槽骨再生,CS/CSn(pDNA-BMP2)-GP组再生显著;钙钴法显示CS/CSn(pDNA-BMP2)-GP组ALP强阳性,CS/CSn-GP组ALP弱阳性,空白对照组为阴性。3组ALP阳性部位着色的平均光密度值比较,CS/CSn(pDNA-BMP2)-GP组> CS/CSn-GP组>空白对照组,两两比较P均﹤0.05。结论 CS/CSn-GP复合修复体系对牙槽骨再生有着积极影响,包载pDNA-BMP-2后,有着更显著的促牙槽骨再生作用。Objective To construct the chitosan/BMP-2 plasmid thermosensitive hydrogel complex CS/CSn(pDNA-BMP2)-GP,and evaluate the effect of this composite system on alveolar bone regeneration in beagle dogs.Methods Periodontitis models were established in the second and third premolars from 4 beagle dogs.All teeth were randomly divided into three groups:CS/CSn-GP group,CS/CSn(pDNA-BMP2)-GP group and blank control group.In the CS/CSn-GP group,CS/CSn-GP was injected.In the CS/CSn(pDNA-BMP2)-GP group,CS/CSn(pDNA-BMP2)-GP was injected.In the blank control group,no material was injected.All animals were sacrificed 8 weeks later.The alveolar bone regeneration in periodontitis was observed by Masson staining.The expression of alkaline phosphatase(ALP)in bone defects was observed by calcium and cobalt staining.Results Masson staining showed that different degrees of alveolar bone regeneration was noted in the CS/CSN-GP and CS/CSn(pDNA-BMP2)-GP groups,and significant regeneration was noted in the CS/CSn(pDNA-BMP2)-GP group.Calcium and cobalt staining revealed strongly-positive ALP in the CS/CSn(pDNA-BMP2)-GP group,weakly-positive ALP in the CS/CSN-GP group,and negative result in the blank control group.The average optical density value in the ALP-positive staining area in the CS/CSn(pDNA-BMP2)-GP group was higher than that in the CS/CSn-GP group,followed by that in the blank control group(all P<0.05).Conclusions CS/CSn-GP composite repair system exerts positive effect on alveolar bone regeneration.Inclusion of pDNA-BMP2 can significantly promote the effect on alveolar bone regeneration.

关 键 词：牙周炎 壳聚糖纳米粒 组织工程支架 骨形态蛋白2 温敏水凝胶 

分 类 号：R781.4[医药卫生—口腔医学]