miR-130b-3p在肾癌中表达及PI3K/AKT途径参与的机制研究  

Expression of miR-130b-3p in renal cell carcinoma and the mechanism of PI3K/AKT pathway involvement

作　　者：谭超 陈海滨[1] 李科技 赵建军[1] TAN Chao;CHEN Hai-bin;LI Ke-ji;ZHAO Jian-jun(The Second Department of Urology,the Affiliated Hospital of Hebei University of Technology,Handan 056002,China)

机构地区：[1]河北工程大学附属医院泌尿外二科,河北邯郸056002

出　　处：《河北医科大学学报》2023年第12期1386-1392,共7页Journal of Hebei Medical University

基　　金：邯郸市科学技术与研究发展项目(19422083011-13)。

摘　　要：目的探究miR-130b-3p调节PI3K/AKT途径对肾癌细胞生物学行为的影响及作用机制。方法临床收集肾癌患者78例,检测肿瘤组织和癌旁组织中miR-130b-3p和PTEN蛋白水平,分析miR-130b-3p与肾癌病理特征的关系。通过相关性分析探究肾癌组织中miR-130b-3p与PTEN蛋白的相关性。786-O细胞分为NC、miR-130b-3p、PTEN和miR-130b-3p+PTEN组,分别转染NC、miR-130b-3p mimic和/或pcDNA 3.1 PTEN来过表达miR-130b-3p和/或PTEN。分别检测各组细胞活力、凋亡率、侵袭能力以及PI3K/AKT通路水平。结果肾癌组织中miR-130b-3p水平显著高于癌旁组织(P<0.05)。miR-130b-3p表达水平与性别、年龄、肿瘤直径无关,高水平的miR-130b-3p与高临床分期和淋巴转移有关(P<0.05)。miR-130b-3p与PTEN靶向结合(P<0.05)。与NC组比较,miR-130b-3p组的PTEN蛋白和凋亡率显著降低,增殖活力、侵袭能力和PI3K/AKT通路显著升高(P<0.05);PTEN组的PTEN蛋白和凋亡率显著升高,增殖活力、侵袭能力和PI3K/AKT通路显著抑制(P<0.05)。miR-130b-3p+PTEN组的PTEN蛋白和凋亡率显著高于miR-130b-3p组且显著低于PTEN组,而增殖活力、侵袭能力和PI3K/AKT通路显著低于miR-130b-3p组且显著高于PTEN组(P<0.05)。结论miR-130b-3p在肾癌组织中上调,高水平的miR-130b-3p与肾癌患者的高临床分期和转移有关,miR-130b-3p可调控PI3K/AKT途径,抑制PTEN蛋白表达,促进肾癌细胞的侵袭并抑制凋亡。Objective To explore the effect and mechanism of miR-130b-3p regulating PI3K/AKT pathway on the biological behavior of renal cancer cells.Methods A total of 78 renal cancer patients were clinically selected.The protein levels of miR-130b-3p and PTEN in tumor tissues and adjacent tissues were detected,and the relationship between miR-130b-3p and pathological features of renal cancer was analyzed.The correlation between miR-130b-3p and PTEN protein in renal cancer tissue was explored by correlation analysis.786-O cells were divided into negative control(NC)group,miR-130b-3p,PTEN and miR-130b-3p+PTEN groups.NC,miR-130b-3p mimic and/or pcDNA 3.1 PTEN were transfected to overexpress miR-130b-3p and/or PTEN,respectively.The cell viability,apoptosis rate,invasion ability and PI3K/AKT pathway level in each group were detected respectively.Results The level of miR-130b-3p in renal cancer tissues was significantly higher than that in adjacent tissues(P<0.05).The expression level of miR-130b-3p was independent of gender,age and tumor diameter.High level of miR-130b-3p was associated with high clinical stage and lymphatic metastasis(P<0.05),with miR-130b-3p targeted binding to PTEN(P<0.05).Compared with the NC group,the PTEN protein and apoptosis rate of the miR-130b-3p group were significantly decreased,and the proliferation activity,invasion ability and PI3K/AKT pathway were significantly increased(P<0.05).In the PTEN group,the PTEN protein and apoptosis rate were significantly increased,while the proliferation activity,invasion ability and PI3K/AKT pathway were significantly inhibited(P<0.05).The PTEN protein and apoptosis rate of the miR-130b-3p+PTEN group were significantly higher than those of the miR-130b-3p group and significantly lower than those of the PTEN group,while the proliferation activity,invasion ability and PI3K/AKT pathway were significantly lower than those of the miR-130b-3p group,and significantly higher than those of the PTEN group(P<0.05).Conclusion miR-130b-3p is upregulated in renal cancer tissues,

关 键 词：肾肿瘤 微小RNA miR-130b-3p 

分 类 号：R737.11[医药卫生—肿瘤]