Effects of Homoharringtonine on the Proliferation and Apoptosis of Melanoma Cells  

作　　者：Rong HU Bin LIU Fan XIAO Xianjing ZENG 

机构地区：[1]Department of Dermatology,Affiliated Hospital of Jinggangshan University,Ji an 343000,China [2]Department of Oncology,Affiliated Hospital of Jinggangshan University,Ji an 343000,China [3]Jinggangshan University,Ji an 343000,China

出　　处：《Medicinal Plant》2023年第6期6-10,共5页药用植物：英文版

基　　金：Supported by the Science and Technology Planning Project of Health Commission of Jiangxi Province(SKJP220229261).

摘　　要：[Objectives]To explore the effects of homoharringtonine(HHT)on the proliferation and apoptosis of melanoma cells.[Methods]Mouse melanoma cell line B16F10 was divided into 5 dfferent dose groups,among which Oμg/mL HHT was as the negative blank group,and the remaining four groups were added with different concentrations of HHT(20,40,80 and i60μg/mL).After B16F10 cells were treated with HHT for 48 h,the growth inhibition rate of B16F10 cells was observed,and the cell cycle was detected.Cyclin D1,MMP-9 and VEGF mRNA and protein levels were detected by qRT-PCR and Western-blot.[Results]Compared with Oμg/mL group,after different concentrations of HHT treated B16F10 cells,the inhibition rate and apoptosis rate of B16F10 cells increased,and Cyclin D1,MMP-9 and VECF mRNA and protein levels in all groups decreased significantly,with statistical significance(P<0.05).[Conclusions]HHT may be involved in the apoptosis of melanoma cells by regulating Cyclin DI level,affecting cell cycle,and inhibiting VEGF and MMP-9 gene protein levels.

关 键 词：Homoharingtonine MELANOMA Matrix metlloproteinases CYELIN 

分 类 号：R285[医药卫生—中药学]