宰后LND处理对藏羊肉线粒体通路细胞凋亡与嫩度的影响  

Effects of LND treatment on postmortem mitochondrial apoptosis and tenderness of Tibetan mutton

作　　者：韩金花 张永胜 陈敬敬 张珍[1] 师希雄[1] HAN Jinhua;ZHANG Yongsheng;CHEN Jingjing;ZHANG Zhen;SHI Xixiong(College of Food Science and Engineering,Gansu Agricultural University,Lanzhou 730070,China)

机构地区：[1]甘肃农业大学食品科学与工程学院,甘肃兰州730070

出　　处：《甘肃农业大学学报》2023年第6期210-216,共7页Journal of Gansu Agricultural University

基　　金：国家自然科学基金资助项目(31760436);甘肃省重点研发计划(20YF3NA014);甘肃省高等学校创新能力提升项目(2020A-049)。

摘　　要：【目的】明确宰后LND处理对藏羊肉成熟过程中线粒体通路细胞凋亡以及肌肉嫩度的影响。【方法】选用甘南欧拉藏羊后腿肉为试验材料,用100μmol/L氯尼达明(Lonidamine,LND)(处理组)和蒸馏水(对照组)以肉液比(m/v)1∶1注射,4℃成熟0、12、24、72、120、168 h,对不同成熟期线粒体膜通透性转换孔(mitochondrial permeablity transition pore,MPTP)开放程度、线粒体膜电位、胞浆Cyt-c氧化还原状态、Caspase-9活性、Caspase-3活性以及肌原纤维小片化指数(MFI)、剪切力指标进行了测定。【结果】在成熟168 h时,LND处理组的吸光值、线粒体膜电位、胞浆Cyt-c还原水平及剪切力值较对照组分别低26.4%(P<0.05)、21.63%、28.4%和15.3%。Caspase-9、Caspase-3活性、MFI值较对照组分别高30.9%、25.1%、6.5%。【结论】研究结果可为宰后藏羊肉的成熟嫩化提供依据。【Objective】To determine the effect of LND treatment on mitochondrial pathway apoptosis and muscle tenderness during postmortem aging of Tibetan mutton.【Method】The hind leg meat of Gannan Oula Tibetan sheep was selected as the test material,and 100μmol/L lonidamine(LND)(treatment group)and distilled water(control group)were injected at a meat-to-liquid ratio(m/v)1∶1,and the degree of MPTP opening,mitochondrial membrane potential,redox state of cytoplasmic Cyt-c,caspase-3 activity,caspase-9 activity,MFI and shear force were measured after aging for 0,12,24,72,120,168 h at 4°C.【Result】After 168 hours of aging,the absorbance,mitochondrial membrane potential,cytoplasmic Cyt-c reduction level,and shear force value of the LND-treated group were 26.4%(P<0.05),21.63%,28.4%,and 15.3%lower than those of the control group,respectively.The activity of caspase-9,caspase-3,and MFI were 30.9%,25.1%,and 6.5%higher,respectively,than that of the control group.【Conclusion】The results provided a basis for postmortem aging and tendering of Tibetan mutton.

关 键 词：藏羊肉 线粒体 嫩度 

分 类 号：TS251.53[轻工技术与工程—农产品加工及贮藏工程]