白灵菇的化学成分及其抗宫颈癌细胞活性研究  

Study on the chemical constituents of Pleurotus tuoliensis and their anti-cervical cancer activity

作　　者：贾月梅 马国需[2,3] 孙照翠 许旭东 石磊岭[3] 贾培松 JIA Yue-mei;MA Guo-xu;SUN Zhao-cui;XU Xu-dong;SHI Lei-ling;JIA Pei-song(Center of Market Supervision and Evaluation of Xinjiang,Urumqi 830011,China;Institute of Medicinal Plant Development,Peking Union Medical College and Chinese Academy of Medical Sciences,Beijing 100193,China;Xinjiang Institute of Chinese Materia Medica and Ethnodrug,Urumqi 830002,China;Institute of Plant Protection,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China)

机构地区：[1]新疆维吾尔自治区市场监督审核评价中心,乌鲁木齐830011 [2]中国医学科学院北京协和医学院药用植物研究所,北京100193 [3]新疆维吾尔自治区中药民族药研究所,乌鲁木齐830002 [4]新疆农业科学院植物保护研究所,乌鲁木齐830091

出　　处：《天然产物研究与开发》2023年第12期2088-2093,共6页Natural Product Research and Development

基　　金：新疆维吾尔自治区自然科学基金(2021D01A109);自治区公益性科研院所基本科研业务费专项(KY2020109)。

摘　　要：为研究白灵菇(Pleurotus tuoliensis)的化学成分及其生物活性,运用ODS、硅胶柱柱色谱、半制备高效液相色谱等方法对其进行分离纯化,结合现代波谱技术与理化性质鉴定化合物结构,从白灵菇提取物中分离得到11个化合物,分别鉴定为镰刀吡酮A(1)、5-羟基二氢镰刀红素C(2)、3-甲基醚镰刀红素(3)、2-acetonyl-3-methyl-7-methoxynaphthazarin(4)、(-)-cyclonerodiol(5)、2-isopropanol-3-methyl-7-methoxy-naphthazarin(6)、1,2-二羟基薄荷内酯(7)、6,7-dihydroxy-3,6-dimethyl-2-isovaleroyl-4,5,6,7-tetrahydrobenzo furan(8)、二氢-3-外胸膜内酯(9)、5-羟甲基糠醛(10)、6,7-dihydroxy-3,6-dimethyl-2-isovaleroyl-4,5,6,7-tetrahydrobenzofuran(11),所有化合物均为首次从白灵菇中分离。对所得化合物进行抗宫颈癌细胞Hela活性筛选,除化合物5、9、10的活性较弱外,其它化合物均显示出较强的抗宫颈癌细胞活性,其中化合物3的活性最强,IC_(50)为3.78μmol/L,具有进一步开发的价值。To study the chemical constituents of Pleurotus tuoliensis and their biological activities.ODS,silica gel column chromatography,semi-preparative high performance liquid chromatography and other methods were used to isolate and purify compounds,and modern spectrum technologies together with physical and chemical methods were applied to identify the structures of those isolated compounds.As a result,11 compounds were isolated from the extract of P.tuoliensis for the first time,named fusarpyrones A(1),5-hydroxydihydrofusarium C(2),3-methyl ether fusarium(3),2-acetonyl-3-methyl-7-methoxy-naphthazarin(4),(-)-cyclonerodiol(5),2-isopropanol-3-methyl-7-methoxy-naphthazarin(6),1,2-dihydroxymentolactone(7),6,7-dihydroxy-3,6-dimethyl-2-isoval eroyl-4,5,6,7-tetrahydrobenzo furan(8),dihydro-3-epi-pleurolactone(9),5-hydroxymethyl furfural(10),6,7-dihydroxy-3,6-dimethyl-2-isovaleroyl-4,5,6,7-tetrahydrobenzofuran(11).Furthermore,all the compounds were screened for anti-cervical cancer activity on Hela cells.The activity results demonstrated that compounds 5,9 and 10 displayed weak inhibitory activity,other compounds showed stronger effects,and compound 3 exhibited the best activity with the IC_(50)value of 3.78μmol/L,which has the value for further research.

关 键 词：白灵菇 化学成分 结构鉴定 抗宫颈癌细胞活性 

分 类 号：R284.2[医药卫生—中药学]