肉源凝固酶阴性葡萄球菌对多环芳烃的消减作用  

Reduction of Polycyclic Aromatic Hydrocarbons by Meat-derived Coagulase-negative Staphylococci

作　　者：刘航航 潘琼 罗慧婷 蔡克周[1] 徐宝才 李沛军[1] LIU Hanghang;PAN Qiong;LUO Huiting;CAI Kezhou;XU Baocai;LI Peijun(School of Food and Biological Engineering,Hefei University of Technology,Hefei 230009,Anhui,China)

机构地区：[1]合肥工业大学食品与生物工程学院,安徽合肥230009

出　　处：《食品研究与开发》2023年第24期9-15,共7页Food Research and Development

基　　金：国家自然科学基金项目(32172235);中央高校基本科研业务费专项资金项目(JZ2022HGTA0317、JZ2023HGQA0111)。

摘　　要：多环芳烃(polycyclic aromatic hydrocarbons,PAHs)是一类食品中广泛存在的危害物,降低其含量有利于提升产品安全性。在模拟体系中考察不同肉源凝固酶阴性葡萄球菌对PAHs的消减作用,筛选出消减PAHs能力最强的菌株,并探讨其作用机理。研究结果表明:在受试菌株中,马胃葡萄球菌(Staphylococcus equorum)E1对模拟体系中PAHs的消减效果最强(P<0.05)。在S.equorum不同细胞组分中,全细胞提取液(whole cell extracts,WCE)和细胞碎片悬液均对PAHs有显著的降低作用(P<0.05)。进一步酶处理发现,WCE中关键消减物质是蛋白类物质;同时,在WCE中检测到了PAHs降解酶活性,这表明S.equorum消减PAHs存在生物降解途径。另一方面,热灭活处理使S.equorum对PAHs消减能力显著提升(P<0.05);通过傅里叶变换红外光谱分析,发现S.equorum的WCE存在与物理吸附PAHs相关的官能团,说明S.equorum消减PAHs存在物理吸附途径。因此,S.equorum可通过生物降解和物理吸附两种作用途径消减PAHs。研究为生物法消减食品中的多环芳烃提供了新途径。polycyclic aromatic hydrocarbons(PAHs)are a ubiquitous food hazard,and reducing their levels is crucial for enhancing product safety.In this simulation system,the abatement effect of coagulase-negative staphylococci on PAHs from various meat sources was investigated,and the strain with the most potent PAH-reducing ability was selected and its mechanism elucidated.The findings indicated that Staphylococcus equorum E1 exhibited the most significant reduction effect on PAHs in the simulated system(P<0.05).Among the different cell components of S.equorum,whole cell extracts(WCE)and cell fragment suspensions had significant decreases in PAHs(P<0.05).Further enzyme treatment revealed that the key subtractive substances in WCE were proteins.At the same time,the activity of PAHs degrading enzyme was detected in WCE,suggesting that S.equorum reduced the biodegradation pathway of PAHs.On the other hand,heat inactivation treatment significantly enhanced the capacity of S.equorum to degrade PAHs(P<0.05).Fourier infrared spectroscopy revealed that WCE of S.equorum possessed functional groups associated with physical adsorption of PAHs,indicating a possible physical adsorption pathway involved in the degradation process.Therefore,S.equorum has the potential to reduce PAHs through biodegradation and physical adsorption,presenting a novel biological approach for reducing PAHs in food.

关 键 词：多环芳烃 凝固酶阴性葡萄球菌 模拟体系 物理吸附 生物降解 

分 类 号：TS201.3[轻工技术与工程—食品科学]