八宝丹联合顺铂抑制胃癌耐药细胞生长和转移的机制研究  

Babaodan Combined with Cisplatin Inhibited Growth and Metastasis of Gastric Cancer Resistant Cells

作　　者：兰炜兰 林久茂 赵锦燕 LAN Weilan;LIN Jiumao;ZHAO Jinyan(Fujian Forestry Vocational&Technical College,Nanping,Fujian 353000,China;Academy of Integrative Medicine,Fujian University of Traditional Chinese Medicine,Fuzhou,Fujian 350122,China;Fujian Key Laboratory of Integrative Medicine on Geriatrics,Fuzhou,Fujian 350122,China;Key Laboratory of Integrative Medicine of Fujian Province University,Fuzhou,Fujian,350122,China)

机构地区：[1]福建林业职业技术学院,福建南平353000 [2]福建中医药大学中西医结合研究院,福建福州350122 [3]福建省中西医结合老年性疾病重点实验室,福建福州350122 [4]中西医结合基础福建省高等学校重点实验室,福建福州350122

出　　处：《福建中医药》2023年第11期15-19,共5页Fujian Journal of Traditional Chinese Medicine

基　　金：福建省自然科学基金项目(2019J01355)。

摘　　要：目的 观察八宝丹(BBD)联合顺铂(DDP)对胃癌耐药细胞(SGC7901/DDP)生长和转移的影响,探讨其抑制SGC7901/DDP细胞生长及转移的机制。方法 将对数生长期SGC7901/DDP细胞消化后接种于96孔板中,分别加入0、1、2、4、6、8、10、16、32μg/mL DDP溶液和0、0.25、0.5、0.75、1.0 mg/mL BBD溶液干预48 h,MTT法筛选DDP和BBD干预浓度分别为2μg/mL和0.25 mg/mL。将SGC7901/DDP细胞分为对照组、DDP组、BBD组和联合组,对照组加入常规培养基,DDP组加入2μg/mL DDP溶液,BBD组加入0.25 mg/mL BBD溶液,联合组加入0.25 mg/mL BBD溶液和2μg/mL DDP溶液,均干预48 h。MTT法检测细胞活力;Transwell实验检测细胞迁移和侵袭能力;黏附实验检测细胞黏附能力;Western blot检测细胞E-钙黏蛋白(E-cadherin)、N-钙黏蛋白(N-cadherin)、波形蛋白(Vimentin)、基质金属蛋白酶(MMP)-9、MMP-2蛋白表达量。结果 与对照组比较,DDP组和BBD组细胞活力明显降低(P<0.05),细胞迁移、侵袭和黏附数均明显减少(P<0.05),N-cadherin、Vimentin、MMP-9蛋白表达量均明显降低(P<0.05),E-cadherin蛋白表达量明显升高(P<0.05);BBD组MMP-2蛋白表达量明显降低(P<0.05)。与DDP组和BBD组比较,联合组细胞活力明显降低(P<0.05),细胞迁移、侵袭和黏附数均明显减少(P<0.05),N-cadherin、Vimentin、MMP-9、MMP-2蛋白表达量均明显降低(P<0.05),E-cadherin蛋白表达量明显升高(P<0.05)。结论 八宝丹联合顺铂可明显抑制SGC7901/DDP细胞的生长和转移,抑制效果较单一应用八宝丹和顺铂明显,其机制可能与其调控胃癌耐药细胞迁移和侵袭相关蛋白有关。Objective:To explore the mechanism of the combination of Babaodan(BBD)and cisplatin(DDP)in inhibiting the growth and metastasis of gastric cancer resistant cells(SGC7901/DDP).Methods:SGC7901/DDP cells with logarithmic growth phase were digested and inoculated into 96 well plates,and added 0,1,2,4,6,8,10,16,32μg/mL DDP solution and 0,0.25,0.5,0.75,1.0 mg/mL BBD solution,respectively intervention for 48 hours.MTT method was used to screen for DDP and BBD intervention concentrations of 2μg/mL and 0.25 mg/mL,respectively.SGC7901/DDP cells were divided into control group,DDP group,BBD group,and combination group.The control group was added to conventional culture medium;the DDP group was added to 2μg/mL DDP solution;the BBD group added 0.25 mg/mL BBD solution;the combination group added 0.25 mg/mL BBD solution and 2μg/mL DDP solution;all intervened for 48 hours.MTT assay was used to detect cell viability;Transwell assay was used to detect cell migration and invasion ability;Adhesion assay was used to detect cell adhesion ability;Western blot was used to detect the protein expression of E-cadherin,N-cadherin,Vimentin,matrix metalloproteinase(MMP)-9,and MMP-2.Results:Compared with the control group,the cell viability of the DDP and BBD groups significantly reduced(P<0.05);the number of cell migration,invasion,and adhesion significantly reduced(P<0.05);the protein expression of N-cadherin,Vimentin,and MMP-9 significantly decreased(P<0.05),while the protein expression of E-cadherin significantly increased(P<0.05).Compared with the control group,the protein expression of MMP-2 in the BBD group significantly decreased(P<0.05).Compared with the DDP and BBD groups,the cell viability of the combination group significantly reduced(P<0.05);the number of cell migration,invasion,and adhesion significantly reduced(P<0.05);the protein expression of N-cadherin,Vimentin,MMP-9,and MMP-2 significantly decreased(P<0.05),while the protein expression of E-cadherin significantly increased(P<0.05).Conclusion:BBD combined with cisplatin c

关 键 词：胃癌 八宝丹 顺铂 上皮间质转化 耐药 转移 迁移 侵袭 

分 类 号：R735.2[医药卫生—肿瘤]