成髓细胞瘤转录因子第2亚型对骨肉瘤细胞恶性行为的影响及其机制  

The effect and molecular mechanism of MYB protooncogene like 2 on the malignant behavior of osteosarcoma cells

作　　者：马海龙 张春雷 张景义[1] 杨昊飞 范丛亮 严磊 海国栋[1] Ma Hailong;Zhang Chunlei;Zhang Jingyi;Yang Haofei;Fan Congliang;Yan Lei;Hai Guodong(Department of Bone Tumor and Osteopathy,Zhengzhou Orthopaedic Hospital,Zhengzhou 450015,China;Department of Orthopaedics,Henan Provincial Chest Hospital,Zhengzhou University,Zhengzhou 450008,China)

机构地区：[1]郑州市骨科医院骨肿瘤骨病科,郑州450015 [2]河南省胸科医院,郑州大学附属胸科医院骨科,郑州450008

出　　处：《中华实验外科杂志》2023年第11期2216-2219,共4页Chinese Journal of Experimental Surgery

基　　金：2020年度河南省医学科技攻关计划联合共建项目(LHGJ20200757)。

摘　　要：目的:探讨成髓细胞瘤转录因子第2亚型(MYBL2),对骨肉瘤细胞增殖、周期、迁移、侵袭和上皮-间充质转化的影响及其机制。方法:选取2017年2月至2023年2月郑州市骨科医院和河南省胸科医院手术治疗的91例骨肉瘤组织及其癌旁组织作为研究对象,采用荧光定量聚合酶链反应(PCR)分析MYBL2表达水平。采用对照慢病毒和MYBL2短发卡RNA(shRNA)慢病毒感染U2OS细胞,建立对照组和MYBL2 KD组细胞,采用噻唑蓝(MTT)法、EdU染色和体外移植瘤实验分析细胞的增殖能力;采用流式细胞术分析两组细胞的周期和凋亡水平;采用Transwell和蛋白质印迹法(Western blot)分析两组细胞的迁移、侵袭和上皮-间充质转化能力。组间计量数据比较采用t检验。结果:癌旁组织MYBL2 mRNA表达水平(1.07±0.16)明显低于骨肉瘤组织(2.57±0.46),差异有统计学意义(t=28.980,P<0.05)。对照组细胞吸光值(A)(1.96±0.08)明显高于MYBL2 KD组(1.57±0.14),差异有统计学意义(t=5.983,P<0.05)。对照组细胞克隆形成率[(65.26±7.06)%]明显高于MYBL2 KD组[(34.17±4.64)%],差异有统计学意义(t=9.015,P<0.05)。对照组细胞体内肿瘤体积[(706.06±94.28)mm 3]明显高于MYBL2 KD组[(422.15±58.66)mm 3],差异有统计学意义(t=6.263,P<0.05)。对照组细胞G 0/G 1期比例[(20.85±2.33)%]明显低于MYBL2 KD组[(20.85±2.33)%],差异有统计学意义(t=3.921,P<0.05)。对照组细胞S期比例[(26.45±2.07)%]明显高于MYBL2 KD组[(22.16±2.94)%],差异有统计学意义(t=2.923,P<0.05)。对照组细胞凋亡率[(2.21±0.85)%]明显低于MYBL2 KD组[(12.90±2.23)%],差异有统计学意义(t=10.950,P<0.05)。对照组细胞迁移数量[(91.15±6.09)个]明显高于MYBL2 KD组[(53.49±12.88)个],差异有统计学意义(t=6.474,P<0.05)。对照组细胞侵袭数量[(71.49±10.52)个]明显高于MYBL2 KD组[(42.73±5.37)个],差异有统计学意义(t=5.996,P<0.05)。对照组细胞上皮细胞标志物E-钙黏蛋白(E-cadherin)[(1.10±0.10)个]明Objective To investigate the effects of MYB protooncogene like 2(MYBL2)on the proliferation,cycle,migration,invasion,and epithelial mesenchymal transition of osteosarcoma cells and its molecular mechanisms.Methods 91 cases of osteosarcoma tissue and its adjacent tissues treated surgically in Zhengzhou Orthopaedic Hospital and Henan Provincial Chest Hospital from February 2017 to February 2023 were selected as the research subjects,and the expression level of MYBL2 was analyzed using fluorescence quantitative polymerase chain reaction(PCR).U2OS cells were infected with control lentivirus and MYBL2 short hairpin RNA(shRNA)lentivirus,and the control group and MYBL2 KD group cells were established.The proliferation ability of the cells was analyzed using methyl thiazolyl tetrazolium(MTT)method,EdU staining,and in vitro tumor transplantation experiment.The cell cycle and apoptosis levels of the two groups of cells were analyzed by flow cytometry.The migration,invasion,and epithelial mesenchymal transformation abilities of the two groups of cells were analyzed by Transwell and Western blotting.The comparison of measurement data between groups was conducted using t-test.Results The expression level of MYBL2 mRNA in adjacent cancer tissues(1.07±0.16)was significantly lower than that in osteosarcoma tissues(2.57±0.46,t=28.980,P<0.05).The absorbance(A)value of the control group cells(1.96±0.08)was significantly higher than that of the MYBL2 KD group cells(1.57±0.14,t=5.983,P<0.05).The cell clone formation rate in the control group[(65.26±7.06)%]was significantly higher than that in the MYBL2 KD group[(34.17±4.64)%,t=9.015,P<0.05].The tumor volume in the control group cells[(706.06±94.28)mm3]was significantly higher than that in the MYBL2 KD group cells[(422.15±58.66)mm3,t=6.263,P<0.05].The proportion of G0/G1 phase cells in the control group[(20.85±2.33)%]was significantly lower than that in the MYBL2 KD group[(20.85±2.33)%,t=3.921,P<0.05].The proportion of S phase cells in the control group[(26.45±2.07)%]was si

关 键 词：成髓细胞瘤转录因子第2亚型 增殖 迁移 周期 转移 

分 类 号：R738.1[医药卫生—肿瘤]