Impact of umbelliprenin-containing niosome nanoparticles on VEGF-A and CTGF genes expression in retinal pigment epithelium cells  

作　　者：Farzad Dastaviz Akram Vahidi Teymoor Khosravi Ayyoob Khosravi Mehdi Sheikh Arabi Abouzar Bagheri Mohsen Rashidi Morteza Oladnabi 

机构地区：[1]Stem Cell Research Center,Golestan University of Medical Sciences,Gorgan 4934174516,Iran [2]Department of Medical Genetics,School of Advanced Technologies in Medicine,Golestan University of Medical Sciences,Gorgan 4934174516,Iran [3]Student Research Committee,Golestan University of Medical Sciences,Gorgan 4934174516,Iran [4]Department of Molecular Medicine,Faculty of Advanced Medical Technologies,Golestan University of Medical Sciences,Gorgan 4934174516,Iran [5]Department of Medical Nanotechnology,Faculty of Advanced Medical Technologies,Golestan University of Medical Sciences,Gorgan 4934174516,Iran [6]Department of Clinical Biochemistry and Genetics,Faculty of Medicine,Mazandaran University of Medical Sciences,Sari 4847191628,Iran [7]Department Pharmacology,Faculty of Medicine,Mazandaran University of Medical Sciences,Sari 4847191628,Iran [8]Gorgan Congenital Malformations Research Center,Golestan University of Medical Sciences,Gorgan 4934174516,Iran

出　　处：《International Journal of Ophthalmology(English edition)》2024年第1期7-15,共9页国际眼科杂志（英文版）

基　　金：Supported by Stem Cell Research Center of Golestan University of Medical Sciences(No.110480).

摘　　要：AIM:To investigate the impact of niosome nanoparticles carrying umbelliprenin(UMB),an anti-angiogenic and anti-inflammatory plant compound,on the expression of vascular endothelial growth factor(VEGF-A)and connective tissue growth factor(CTGF)genes in a human retinal pigment epithelium(RPE)-like retina-derived cell line.METHODS:UMB-containing niosomes were created,optimized,and characterized.RPE-like cells were treated with free UMB and UMB-containing niosomes.The IC_(50)values of the treatments were determined using an MTT assay.Gene expression of VEGF-A and CTGF was evaluated using real-time polymerase chain reaction after RNA extraction and cDNA synthesis.Niosomes’characteristics,including drug entrapment efficiency,size,dispersion index,and zeta potential were assessed.Free UMB had an IC_(50)of 96.2μg/mL,while UMB-containing niosomes had an IC_(50)of 25μg/mL.RESULTS:Treatment with UMB-containing niosomes and free UMB resulted in a significant reduction in VEGF-A expression compared to control cells(P=0.001).Additionally,UMB-containing niosomes demonstrated a significant reduction in CTGF expression compared to control cells(P=0.05).However,there was no significant reduction in the expression of both genes in cells treated with free UMB.CONCLUSION:Both free UMB and niosome-encapsulated UMB inhibits VEGF-A and CTGF genes expression.However,the latter demonstrates significantly greater efficacy,potentially due to the lower UMB dosage and gradual delivery.These findings have implications for anti-angiogenesis therapeutic approaches targeting age-related macular degeneration.

关 键 词：umbelliprenin NIOSOME age-related macular degeneration vascular endothelial growth factor-A connective tissue growth factor 

分 类 号：R77[医药卫生—眼科]