添加植物乳杆菌、布氏乳杆菌对杂交狼尾草青贮发酵品质及有氧稳定性的影响  

Effects of Adding Lactiplantibacillus plantarum and Lactobacillus buchneri on Silage Fermentation Quality and Aerobic Stability of Pennisetum glaucum×purpureum

作　　者：高龙 张娟 戈辰妍 刘宽 唐嘉祺 石神炳 阳伏林 周晶 GAO Long;ZHANG Juan;GE Chen-yan;LIU Kuan;TANG Jia-qi;SHI Shen-bing;YANG Fu-lin;ZHOU Jing(College of Animal Sciences(College of Bee Science),Fujian Agriculture and Forestry University,Fuzhou,Fujian Province 350002,China;Nanping Jianyang District Jixiang Animal Husbandry Co.Ltd,Nanping,Fujian Province 354207,China;China National Engineering Research Center of JUNCAO Technology,Fujian Agriculture and Forestry University,Fuzhou,Fujian Province 350002,China)

机构地区：[1]福建农林大学动物科学学院(蜂学学院),福建福州350002 [2]南平市建阳区吉翔牧业有限公司,福建南平354207 [3]福建农林大学国家菌草工程技术研究中心,福建福州350002

出　　处：《草地学报》2023年第12期3867-3875,共9页Acta Agrestia Sinica

基　　金：福建省科技特派员后补助项目(2022S2071);福建农林大学杰出青年科研人才计划项目(xjq201817)资助。

摘　　要：为探究植物乳杆菌、布氏乳杆菌对杂交狼尾草(Pennisetum glaucum×purpureum)青贮发酵品质及有氧稳定性的影响,本试验以杂交狼尾草为试验材料,设置对照组(CK组,无添加)、植物乳杆菌(Lactiplantibacillus plantarum)组(LP组,1×10^(6)cfu·g^(-1)FW)、布氏乳杆菌(Lactobacillus buchneri)组(LB组,1×10^(6)cfu·g^(-1)FW)、植物乳杆菌和布氏乳杆菌组(LPLB组,5×10^(5)cfu·g^(-1)FW LP+5×10^(5)cfu·g^(-1)FW LB),厌氧发酵60天(d)后,测定其营养成分、发酵品质和有氧稳定性。结果表明:与CK组相比,各处理组粗蛋白(CP)、乳酸(LA)和乙酸(AA)含量显著增加(P<0.05),pH值和丁酸(BA)含量显著下降(P<0.05);在有氧暴露阶段,各处理组乳酸(LA)含量显著增加(P<0.05),氨态氮含量显著降低(P<0.05),LB和LPLB组在有氧暴露9天时,酵母菌和好氧菌数量(P<0.05)显著降低,乳酸菌数量显著增加(P<0.05);用隶属函数法进行综合价值评定,LPLB组青贮效果最佳。The aim of study was to explore the effects of Lactiplantibacillus plantarum and Lactobacillus buchneri on the silage fermentation quality and aerobic stability of Pennisetum glaucum×purpureum.Four groups were established as follows:CK group(no addition),Lactobacillus plantarum group(LP group,1×10^(6)cfu·g^(-1)FW),Lactobacillus buchneri group(LB group,1×10^(6)cfu·g^(-1)FW),and Lactiplantibacillus plantarum and Lactobacillus buchneri group(LPLB group,5×10^(5)cfu·g^(-1)LP+5×10^(5)cfu·g^(-1)LB).After 60 days of fermentation,the fermentation quality and aerobic stability of all the Pennisetum glaucum×purpureum silage were measured.The results showed that:Compared with the CK group,the addition of Lactic acid bacteria significantly improved the crude protein(CP)content in all treatment groups(P<0.05).The pH value and butyric acid(BA)content of Lactic acid bacteria treatment were significantly lower than those of CK group(P<0.05),while the contents of lactic acid(LA)and acetic acid(AA)were significantly higher than those of CK group(P<0.05).During the aerobic exposure,the content of LA was significantly higher than that of CK group(P<0.05).The content of ammonia nitrogen was lower than that of CK group(P<0.05).After 9 days of aerobic exposure,yeast and aerobic bacteria numbers significantly reduced,while lactic acid bacteria numbers significantly increased in LB and LPLB groups(P<0.05).The comprehensive value evaluation by the membership function method found that LPLB group had the best comprehensive performance of silage effect.

关 键 词：杂交狼尾草 植物乳杆菌 布氏乳杆菌 发酵品质 有氧稳定性 

分 类 号：S816.53[农业科学—饲料科学]