双氢青蒿素对胃癌SGC-7901细胞凋亡和侵袭能力的影响  

Effects of Dihydroartemisinin on Apoptosis and Invasion of Gastric Cancer SGC-7901 Cells

作　　者：刘师兵[1] 董长松[2] 朱文赫[1] 于洋[1] 林松 高金昌 LIU Shibing;DONG Changsong;ZHU Wenhe;YU Yang;LIN Song;GAO Jinchang(Research Laboratory of Jilin Medical University,Jilin City,Jilin Province 132013;Department of Gastroenterology,465 Affiliated Hospital of Jilin Medical Univer-sity,Jilin City,Jilin Province 132013,China)

机构地区：[1]吉林医药学院科研实验室,吉林吉林132013 [2]吉林医药学院附属四六五医院消化内科,吉林吉林132013

出　　处：《吉林医药学院学报》2024年第1期5-9,共5页Journal of Jilin Medical University

基　　金：吉林省中医药科技项目(2022085)。

摘　　要：目的初步探讨检测双氢青蒿素(Dihydroartemisinin,DHA)对胃癌SGC-7901细胞凋亡和侵袭能力作用的可能机制。方法以胃癌SGC-7901细胞为研究对象,实验分为空白组和DHA低(40μmol/L)、中(80μmol/L)、高剂量组(120μmol/L)。CCK-8检测法检测不同浓度DHA对SGC-7901细胞增殖的影响;Hoechst 33258染色法观察各组细胞形态变化;采用流式细胞术检测不同给药组的细胞凋亡情况;Transwell法检测不同浓度DHA对SGC-7901细胞侵袭能力的影响;Western blot检测Bcl-2、Bax、cleaved caspase-3和VEGF蛋白表达。结果CCK-8检测法显示,与空白组相比,DHA各浓度组的细胞增殖受到明显抑制,且随着药物浓度的增加呈现剂量依赖性;Hoechst 33258染色结果显示,DHA各浓度组的SGC-7901细胞核内出现明显固缩,且着色增强;流式细胞术结果显示,给药组凋亡细胞数量明显增多,且与给药浓度成正比;Transwell检测结果显示,DHA各浓度组中穿透基质膜的细胞数量明显下降;Western blot检测结果显示,与空白组相比,DHA各浓度组Bax和cleaved caspase-3蛋白表达显著上升(P<0.05),Bcl-2、VEGF蛋白表达显著降低(P<0.05)。结论DHA可能通过调控Bcl-2、Bax、cleaved caspase-3表达,抑制SGC-7901细胞增殖并诱导细胞凋亡,通过调控VEGF表达降低SGC-7901细胞的侵袭能力。Objective To explore the possible mechanism of detecting the effect of dihydroartemisinin(DHA)on the apoptosis and inva-sion ability of gastric cancer SGC-7901 cells.Methods Using gastric cancer SGC-7901 cells as the research object,the experiment was divided into a blank group and a low(40μmol/L),medium(80μmol/L),and high-dose group(120μmol/L)of DHA.CCK-8 de-tection method was used to detect the effect of different concentrations of DHA on the proliferation of SGC-7901 cells;Observe the mor-phological changes of cells in each group using Hoechst 33258 staining method;Flow cytometry was used to detect cell apoptosis in differ-ent treatment groups;Transwell method was used to detect the effect of different concentrations of DHA on the invasion ability of SGC-7901 cells;Western blot was used to detect the expression of Bcl-2,Bax,cleared caspase-3,and VEGF proteins.Results The CCK-8 detection method showed that compared with the blank group,the cell proliferation of each concentration group of DHA was significantly inhibited,and showed a dose-dependent relationship with the increase of drug concentration;The Hoechst 33258 staining results showed significant pyknosis and enhanced staining in the nuclei of SGC-7901 at various concentrations of DHA;The results of flow cytometry showed that the number of apoptotic cells in the treatment group increased significantly and was proportional to the concentration of the drug;The Transwell test results showed that the number of cells penetrating the matrix membrane significantly decreased in each concen-tration group of DHA;Western blot analysis showed that compared with the blank group,the expression of Bax and cleared caspase-3 pro-teins in the DHA concentration groups significantly increased(P<0.05),while the expression of Bcl-2 and VEGF proteins significantly decreased(P<0.05).Conclusion DHA may inhibit SGC-7901 cell proliferation and induce apoptosis by regulating the expression of Bcl-2,Bax,and cleared caspase-3,and reduce the invasive ability of SGC-7901 cells by reg

关 键 词：双氢青蒿素 胃癌 凋亡 侵袭能力 

分 类 号：R735.7[医药卫生—肿瘤] R285.5[医药卫生—临床医学] R996.3