山柰酚对溴氰菊酯诱导卤虫氧化应激的保护作用  

Protective Effect of Kaempferol on Deltamethrin-Induced Oxidative Stress in Brine Shrimp Artemia

作　　者：马丹丹 隋丽英 周庆礼[1] 郭庆彬 潘娜敏 李贞景[1] MA Dandan;SUI Liying;ZHOU Qingli;GUO Qingbin;PAN Namin;LI Zhenjing(College of Food Science and Engineering,Tianjin University of Science&Technology,Tianjin 300457,China;College of Marine and Environmental Sciences,Tianjin University of Science&Technology,Tianjin 300457,China)

机构地区：[1]天津科技大学食品科学与工程学院,天津300457 [2]天津科技大学海洋与环境学院,天津300457

出　　处：《天津科技大学学报》2023年第6期12-18,80,共8页Journal of Tianjin University of Science & Technology

基　　金：天津市科技计划项目(22YDTPJC00370);天津市教委科研计划项目(2018KJ093)。

摘　　要：为了探究山柰酚(kaempferol,Ka)对溴氰菊酯(deltamethrin,DEL)暴露卤虫抗氧化应激能力的影响,以新疆维吾尔自治区艾比湖孤雌生殖卤虫(Artemiaparthenogenetica)为研究对象,分析溴氰菊酯和山柰酚对卤虫存活率、孵化率、体长、抗氧化酶活性、脂质过氧化物和Nrf2信号通路的影响。结果表明:溴氰菊酯对卤虫的半致死浓度(LC50)为0.210μg/L,0.1 mg/mL山柰酚能够有效提高卤虫的孵化率、存活率和体长。山柰酚能够显著抑制溴氰菊酯诱导卤虫的活性氧(ROS)水平和丙二醛(MDA)含量,提高超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和过氧化氢酶(CAT)的活性,上调Nrf2信号通路中关键基因SOD、Nrf2、GST、NQO1、HO-1和CAT的表达量,下调基因Keap1的表达量(P<0.05)。因此,山柰酚能够有效缓解溴氰菊酯诱导的氧化应激损伤,主要作用机制是提高卤虫抗氧化酶的活性,调节Nrf2通路中关键基因表达。In order to explore the effect of kaempferol(Ka)on the antioxidant stress of Artemia exposed to deltamethrin(DEL),in this study we analyze the effects of DEL and Ka on the survival rate,hatching percentage,body length,antioxidant enzyme activities,lipid peroxide and Nrf2 signal pathway in Artemia parthenogenetic in Lake Ebinur,Xinjiang.The result showed that the LC50 of DEL to Artemia was 0.210μg/L,and 0.1 mg/mL Ka exerted its antioxidative effect on the hatching percentage,survival rate and body length of Artemia.Furthermore,Ka could significantly inhibit deltamethrininduced ROS levels and malondialdehyde(MDA)content in Artemia,improve the activities of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px)and catalase(CAT),increase the expression levels of key genes SOD,Nrf2,GST,NQO1,HO-1 and CAT in the Nrf2 signaling pathway,and decrease the expression levels of Keap1(P<0.05).Therefore,the results of this study show that Ka can effectively alleviate the oxidative stress damage induced by DEL.The main mechanism is to activate the antioxidant enzymes activities of Artemia and regulate the expression of key genes in Nrf2 pathway.

关 键 词：卤虫 山柰酚 溴氰菊酯 氧化应激 

分 类 号：S963.16[农业科学—水产养殖]