lncRNA Meg3在全反式维甲酸和转化生长因子β3影响小鼠腭突间充质细胞增殖过程中的作用  

Role of lncRNA Meg3 in proliferation of mouse embryonic palatal mesenchymal cells treated by all-trans retinoic acid or transforming growth factor β3

作　　者：刘小转 张军喜 余增丽 王国旭 何志东 宋帅星 李雪 LIU Xiaozhuan;ZHANG Junxi;YU Zengli;WANG Guoxu;HE Zhidong;SONG Shuaixing;LI Xue(Clinical Medical Research Center,Henan Provincial People′s Hospital,Zhengzhou 450003;NHC Key Laboratory of Birth Defects Prevention,Henan Key Laboratory of Population Defects Prevention,Zhengzhou 450002;Department of Nutrition and Food Hygiene,College of Public Health,Zhengzhou University,Zhengzhou 450001)

机构地区：[1]河南省人民医院临床医学研究中心,郑州450003 [2]国家卫生健康委员会出生缺陷预防重点实验室,河南省人口缺陷干预技术研究重点实验室,郑州450002 [3]郑州大学公共卫生学院营养与食品卫生学教研室,郑州450001

出　　处：《郑州大学学报（医学版）》2023年第6期763-769,共7页Journal of Zhengzhou University(Medical Sciences)

基　　金：国家自然科学基金项目(81801547);河南省科技攻关省部共建重点项目(SBGJ202102014);国家卫生健康委员会出生缺陷预防重点实验室&河南省人口缺陷干预技术研究重点实验室开放基金项目(ZD202203)。

摘　　要：目的:探索长链非编码RNA(lncRNA)Meg3在全反式维甲酸(atRA)和转化生长因子β3(TGF-β3)影响胎鼠腭突间充质细胞增殖过程中的作用。方法:分离、培养妊娠13 d胎鼠腭突间充质细胞。EdU法检测对照组(未处理)、atRA组(5μmol/L atRA)和TGF-β3组(10μg/L TGF-β3)处理48 h腭突间充质细胞增殖能力的变化。荧光原位杂交和实时荧光定量PCR法检测上述3组Meg3的定位和相对表达量。利用EdU实验检测Meg3 siRNA转染前后atRA和TGF-β3对腭突间充质细胞增殖的影响。结果:与对照组相比,atRA处理48 h可抑制腭突间充质细胞的增殖,TGF-β3处理48 h可促进腭突间充质细胞增殖(P<0.05)。与对照组相比,atRA可促进腭突间充质细胞中Meg3的表达,TGF-β3可抑制Meg3的表达(P<0.05)。Meg3基因沉默后,与对照组相比,Meg3 siRNA组、atRA和TGF-β3处理组胎鼠腭突间充质细胞增殖能力均增强(P<0.05)。结论:atRA对胎鼠腭突间充质细胞的增殖抑制作用可能主要通过Meg3介导,而TGF-β3对腭突间充质细胞增殖的促进作用可能与Meg3基因无直接关系。Aim:To explore the role of long non-coding RNA(lncRNA) Meg3 in the proliferation of mouse embryonic palate mesenchymal cells treated by all-trans retinoic acid(atRA) or transforming growth factor β3(TGF-β3).Methods:Mouse embryonic palate mesenchymal cells were isolated and cultured on gestation day 13.EdU assay was used to detect the proliferation ability of mouse embryonic palate mesenchymal cells after treatment with 5 μmol/L atRA(atRA group) or 10 μg/L TGF-β3(TGF-β3 group) for 48 hours.The cells not treated were used as control.The localization and relative expression of Meg3 in the cells of the above 3 groups were detected by fluorescence in situ hybridization and real-time fluorescence quantification PCR.EdU assay was used to detect the effects of 5 μmol/L atRA and 10 μg/L TGF-β3 on the proliferation of mouse embryonic palate mesenchymal cells before and after Meg3 siRNA transfection.Results:Compared with control,atRA significantly inhibited the proliferation of mouse embryonic palate mesenchymal cells,while TGF-β3 significantly promoted the proliferation;besides,atRA prompted the expression of Meg3 in mouse embryonic palate mesenchymal cells,while TGF-β3 inhibited the expression of Meg3(P<0.05).After Meg3 gene silencing,compared with control group,the proliferation of mouse embryonic palate mesenchymal cells in the Meg3 siRNA group,atRA group and TGF-β3 group was significantly promoted(P<0.05).Conclusion:The inhibitive effects of atRA on palatal mesenchymal cell proliferation may be primarily mediated by Meg3,while the effects of TGF-β3 on the proliferation of palatal mesenchymal cells may not directly relate to Meg3 gene.

关 键 词：长链非编码RNA Meg3 腭裂 腭突间充质细胞 全反式维甲酸 转化生长因子Β3 小鼠 

分 类 号：R782[医药卫生—口腔医学]