昆虫杆状病毒及其应用研究进展  

Research progress in the application of insect baculovirus

作　　者：梁振普[1,2] 陈阳光 杜俊阳 曹瀚文 张小霞 代金平[2] 杨新平[2] 苏萍[3] LIANG Zhenpu;CHEN Yangguang;DU Junyang;CAO Hanwen;ZHANG Xiaoxia;DAI Jinping;YANG Xinping;SU Ping(College of Life Science,Henan Agricultural University,Zhengzhou 450002,China;Institute of Microbiology Application,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China;Agricultural Science Research Institute of the 14th Division,Xinjiang Production and Construction Corps,Kunyu 848116,China)

机构地区：[1]河南农业大学生命科学学院,河南郑州450002 [2]新疆农业科学院微生物应用研究所,新疆乌鲁木齐830091 [3]新疆生产建设兵团第十四师农业科学研究所,新疆昆玉848116

出　　处：《河南农业大学学报》2023年第6期916-923,共8页Journal of Henan Agricultural University

基　　金：新疆维吾尔自治区重点研发任务专项项目(2022B02019);南疆重点产业创新发展支撑计划项目(2022DB026)。

摘　　要：杆状病毒是一类具有双链、环状DNA的病毒,因其在生物防治方面具有宿主专一性强、对人畜无害、对环境安全和可自然传播等特点,而得到人们广泛的关注。本文概述了昆虫杆状病毒特征和作为表达系统的应用。野生型昆虫杆状病毒具有杀虫速度慢、稳定性差和宿主域窄等缺点,因此本文还介绍了通过基因工程改造野生型昆虫杆状病毒,以提高其作用效率和扩大杀虫谱的方法,同时对杆状病毒的应用前景做出了展望。Baculovirus is a kind of virus with double-stranded and circular DNA,which has attracted wide attention because of its characteristics in biocontrol field with host specificity,harmless to humans and animals,environmental safety and natural transmission.In this paper,the characteristics of insect baculovirus and its application as an expression system are reviewed.Wild-type insect baculovirus has the disadvantages of slow killing speed,poor stability and narrow host domain,so this paper also intro-duces the method of modifying wild-type insect baculovirus by genetic engineering to improve its effi-ciency and expand its insecticidal spectrum.At the same time,the application prospect of baculovirus is prospected.

关 键 词：杆状病毒 表达系统 杀虫剂 基因工程 

分 类 号：S435[农业科学—农业昆虫与害虫防治]