菌-藻体系中无机碳源对小球衣藻生长和聚集的影响研究  

Inorganic carbon sources significantly promoted growth and aggregation of Chlamydomonas microsphaera in bacterial-algal system

作　　者：吴雪菲 陈国炜[1] 方志鹏 贾伟 刘丽[1] WU Xuefei;CHEN Guowei;FANG Zhipeng;JIA Wei;LIU Li(School of Civil and Hydraulic Engineering,Hefei University of Technology,Hefei 230009,China)

机构地区：[1]合肥工业大学土木与水利工程学院,安徽合肥230009

出　　处：《合肥工业大学学报（自然科学版）》2023年第12期1595-1601,1615,共8页Journal of Hefei University of Technology：Natural Science

基　　金：国家自然科学基金资助项目(42277298)

摘　　要：为了提高微藻生长速率、实现低成本高效收集,文章以小球衣藻(Chlamydomonas microsphaera)为研究对象,探究无机碳源对菌-藻体系中微藻生长和聚集的影响。结果表明:无机碳源能够显著提高菌-藻体系中微藻生物量,当初始菌藻数量比为15∶1时,在低碳源(37.6 mg/L)水平下,经过6 d培养后微藻生物量可达1.255×10^(6)个/mL,较纯藻培养体系增加30%;当碳源质量浓度升高至188.0 mg/L时,微藻数量较纯藻培养体系增加68%,可达1.681×10^(6)个/mL;菌-藻体系也有助于微藻自聚集,随着无机碳源质量浓度增加,其聚集效果变得更明显,聚集率最高可达66%,远高于纯培养体系中的27%。研究结果可为微藻综合利用研究提供参考。In order to improve the growth rate of microalgae and achieve low-cost and high-efficiency collection,this paper takes Chlamydomonas microsphaera as the research object to explore the effects of inorganic carbon sources on the growth and aggregation of microalgae in the bacterial-algal system.The results showed that inorganic carbon sources could significantly increase the biomass of microalgae in the bacterial-algal system.When the initial ratio of bacteria to algae was 15∶1,the biomass of microalgae could reach 1.255×10^(6) cells/mL after six days of culture under the low carbon source(37.6 mg/L),which was 30% higher than that in the pure algal culture system.When the carbon source mass concentration increased to 188.0 mg/L,the number of microalgae increased by 68% compared with that of pure algal culture system,reaching 1.681×10^(6) cells/mL.The bacterial-algal system also contributed to the self-aggregation of microalgae.With the increase of the mass concentration of inorganic carbon source,the aggregation effect became more obvious,and the aggregation rate was up to 66%,which was much higher than 27%in the pure culture system.The results can provide reference for the comprehensive utilization of microalgae.

关 键 词：无机碳源 菌-藻体系 微藻生长 微藻聚集 

分 类 号：X172[环境科学与工程—环境科学]