微塑料和镉复合污染对狼尾草根际土壤微生物群落结构和功能的影响  

Effects of Combined Pollution of Microplastics and Cadmium on Microbial Community Structure and Function of Pennisetum hydridum Rhizosphere Soil

作　　者：段莉阳 张玉 任学敏[1] 李玉英[1] 张英君[2] 张浩 韩辉 陈兆进 DUAN Li-yang;ZHANG Yu;REN Xue-min;LI Yu-ying;ZHANG Ying-jun;ZHANG Hao;HAN Hui;CHEN Zhao-jin(International Joint Laboratory of Watershed Ecological Security,Collaborative Innovation Center of Water Security for Water Source Region of Middle Route Project of South-North Water Diversion in Henan Province,College of Water Resource and Environment Engineering,Nanyang Normal University,Nanyang 473061,China;School of Life Science and Agricultural Engineering,Nanyang Normal University,Nanyang 473061,China)

机构地区：[1]南阳师范学院水资源与环境工程学院,河南省南水北调中线水源区流域生态安全国际联合实验室,南水北调中线水源区水安全河南省协同创新中心,南阳473061 [2]南阳师范学院生命科学与农业工程学院,南阳473061

出　　处：《环境科学》2023年第12期6973-6981,共9页Environmental Science

基　　金：国家自然科学基金项目(U2004145,41601332);河南省高校科技创新人才项目(23HASTIT018);河南省重点研发专项(221111520600);河南省中央引导地方科技发展资金项目(Z20221343035);河南省高等学校重点科研项目(23A610003)。

摘　　要：微塑料和重金属复合污染对植物生长和根际微生物群落和功能的影响目前尚未清晰.以狼尾草为实验材料进行盆栽模拟试验,研究重金属镉(Cd)与不同种类MPs(PE、PS)、粒径(13μm和550μm)、质量分数(0.1%和1%)的微塑料复合污染对狼尾草生长、重金属积累和根际微生物群落功能的影响.结果表明,MPs和Cd复合污染条件下整体呈现对植物生长胁迫增加、Cd含量和积累量降低的趋势.MPs和Cd复合污染能改变细菌群落组成,降低细菌多样性,其中550μm 0.1%PE+Cd处理组ACE指数和Chao1指数降低最显著.与单一Cd污染相比,不同MPs种类、质量分数和粒径的MPs添加能改变新陈代谢、氨基酸的转运和代谢、能量生成和转换等功能组的基因丰度,显著影响狼尾草根际土壤细菌的功能.研究采用宏基因组学的方法分析了MPs和Cd复合污染对狼尾草根际细菌群落和功能的影响,可为MPs重金属复合污染的生态毒理效应及其生物修复提供基础数据和科学依据.The combined pollution of microplastics and heavy metals can potentially interact.This may have an important impact on the growth and development of plants and the rhizosphere microbial community and function.In this study,the effects of heavy metal cadmium combined with different types of microplastics(PE and PS),different particle sizes(13μm and 550μm),and different concentrations(0.1%and 1%)on Pennisetum hydridum growth were studied under pot conditions.The results showed that the effects of the combined pollution of MPs and Cd on plant dry weight and Cd accumulation varied with different types,concentrations,and particle sizes of MPs,and the combined pollution stress increased,whereas the Cd content and Cd accumulation decreased.Metagenomic analysis showed that the combined contamination of MPs and Cd could change the composition of the bacterial community and reduce bacterial diversity,among which the ACE index and Chao1 index in the 550μm 0.1%PE+Cd treatment group were the most significant.Metagenomic analysis of microbial species function showed that the main functional groups were metabolism,amino acid transport and metabolism,energy generation and conversion,and signal transduction mechanisms.Compared with that under single Cd pollution,the addition of MPs could change the gene abundance of functional groups such as metabolism,amino acid transport and metabolism,and energy generation and conversion,and the effects of different MPs types,concentrations,and particle sizes varied.In this study,metagenomics and amplification sequencing were used to analyze the effects of the combined pollution of MPs and Cd on the bacterial community and function in P.hydridum in order to provide basic data and scientific basis for the ecotoxicological effects of the combined heavy metal pollution of MPs and its biological remediation.

关 键 词：微塑料(MPs) 镉(CD) 狼尾草 宏基因组 群落结构和功能 

分 类 号：X172[环境科学与工程—环境科学]