广金钱草不同极性萃取物体外降脂及降血糖活性的比较  

Comparison of the in Vitro Lipid-lowering and Hypoglycemic Activities of Desmodium styracifolium Extracts with Different Polarities

作　　者：张会香[1] 汤霞利 林军全 陈海珊[2] 林伟国 梁筱彬 李霞[1] 关媛 ZHANG Huixiang;TANG Xiali;LIN Junquan;CHEN Haishan;LIN Weiguo;LIANG Xiaobin;LI Xia;GUAN Yuan(College of Chemistry and Bioengineering,Guilin University of Science and Technology,Guilin 541006,China;Guangxi Institute of Botany,Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences,Guilin 541006,China;Guangxi Zhongheng Chinese Herbal Medicine Industry Development Co.Ltd.,Wuzhou 543000,China)

机构地区：[1]桂林理工大学化学与生物工程学院,广西桂林541006 [2]广西壮族自治区中国科学院广西植物研究所,广西桂林541006 [3]广西中恒中药材产业发展有限公司,广西梧州543000

出　　处：《现代食品科技》2023年第12期192-198,共7页Modern Food Science and Technology

基　　金：国家自然科学基金青年基金(32000253);国家自然科学基金地区基金(31860251);广西高校中青年教师科研基础能力提升项目(2020KY06018)。

摘　　要：该研究旨在比较广金钱草乙醇提取物及其各萃取相的体外降脂及降血糖活性。以φ=75%乙醇为溶剂,回流提取得到广金钱草醇提物,经石油醚、乙酸乙酯、正丁醇、水依次萃取,利用比色法检测乙醇提取物及各萃取相中总黄酮、总酚含量,并根据胰脂肪酶和胆固醇酯酶的抑制率评价乙醇提取物及其各萃取相的体外降脂活性,对α-葡萄糖苷酶和α-淀粉酶的抑制率评价体外降血糖活性。研究结果显示乙酸乙酯萃取相中总黄酮和总酚含量最高,分别为154.57 mg/g和34.27 mg/g。乙醇提取物对胆固醇酯酶和α-葡萄糖苷酶的抑制活性最高,抑制率分别为53.24%和68.52%;正丁醇萃取相对胰脂肪酶表现出最高的抑制活性,抑制率达到77.39%。石油醚萃取相对α-淀粉酶的抑制作用最强,抑制率达到78.60%。研究表明,广金钱草乙醇提取物及其各萃取相具有显著的降脂和降血糖活性,可为其深入研究药理活性提供参考依据。The in vitro lipid-lowering and hypoglycemic activities of the ethanol extract and derived extraction phases from Desmodium styracifolium were compared.The ethanol extract was obtained by reflux extraction with 75% ethanol as the solvent,which was sequentially fractionated using petroleum ether,ethyl acetate,n-butanol,and water.The total flavonoids and total phenolic contents of the ethanol extract and each derived extraction phase were determined by the colorimetric method.The in vitro lipid-lowering activities of the ethanol extract and each derived extraction phase were determined based on the inhibition rates of pancreatic lipase and cholesterol esterase.The in vitro hypoglycemic activities were determined based on the inhibition rates of α-glucosidase and α-amylase.The results showed that the ethyl acetate phase had the highest total flavonoids and total phenolic contents(154.57 mg/g and 34.27 mg/g,respectively).The ethanol extract had the highest inhibitory activity against cholesterol esterase and α-glucosidase(inhibition rates:53.24% and 68.52%,respectively).The n-butanol phase showed the highest pancreatic lipase inhibitory activity(inhibition rate:77.39%).The petroleum ether phase had the strongest inhibitory effect onα-amylase(inhibition rate:78.60%).The research revealed that the ethanol extract and derived extraction phases from Desmodium styracifolium possessed significant lipid-lowering and hypoglycemic activities,which provides a reference for in-depth research on its pharmacological activities.

关 键 词：广金钱草 乙醇提取物 降脂活性 降血糖活性 

分 类 号：R285[医药卫生—中药学]