LncRNA RP5-919F19在胃癌组织中的表达及其与胃癌侵袭转移的相关性  

Expression of LncRNA RP5-919F19 in gastric cancer and its correlation with invasion and metastasis of gastric cancer

作　　者：宗焕波 武菲 黄兆栋 戚春厚 李善凯 郝红军 王从晓 Zong Huanbo;Wu Fei;Huang Zhaodong;Qi Chunhou;Li Shankai;Hao Hongjun;Wang Congxiao(Department of Intervention,Linyi Central Hospital,Linyi 276400,China;Department of Gastroenterology,Linyi Central Hospital,Linyi 276400,China;Department of Intervention,Affiliated Hospital of Qingdao University,Qingdao 266000,China)

机构地区：[1]临沂市中心医院介入科,临沂276400 [2]临沂市中心医院消化科,临沂276400 [3]青岛大学附属医院介入科,青岛266000

出　　处：《中华内分泌外科杂志》2023年第6期675-680,共6页Chinese Journal of Endocrine Surgery

基　　金：山东省医药卫生科技发展计划项目(2018ws393)。

摘　　要：目的研究长非编码RNA(long non-coding RNA,Lnc RNA)RP5-919F19在胃癌组织中的表达及与胃癌侵袭转移的相关性。方法收集临沂市中心医院2020年1月到2022年1月手术取下的非肿瘤胃黏膜(距癌组织3 cm以上)和胃腺癌组织。应用TRIzol试剂盒提取细胞和组织总RNA,应用反转录试剂盒将RNA反转录为cDNA,应用实时定量PCR试剂盒进行定量分析。应用SGC-7901和AGS人胃癌细胞,构建RP5-919F19敲减和过表达模型,应用CCK-8实验确认细胞增殖能力,应用Transwell侵袭实验确认胃癌细胞的侵袭能力。结果对79例胃癌组织和癌旁正常组织中RP5-919F19表达检测,发现RP5-919F19在胃癌组织中的相对表达量为1.51±0.05,明显高于癌旁正常组织的0.82±0.04(P<0.05)。对不同病理情况患者的RP5-919F19水平进行对比分析,分析结果显示患者不同TNM分期、是否出现远处转移、淋巴结转移和不同浸润深度患者的RP5-919F19表达差异存在统计学意义(P<0.05),不同肿瘤大小、年龄和性别患者RP5-919F19表达差异无统计学意义(P>0.05)。对AGS胃癌细胞转染RP5-919F19过表达和对照质粒,对细胞RP5-919F19效率进行检测,检测结果发现,过表达组细胞的RP5-919F19表达水平为1.83±0.14,相对对照组的0.82±0.05高(P<0.05),对SGC-7901胃癌细胞转染RP5-919F19敲除和对照载体,对细胞RP5-919F19效率进行检测,检测结果发现,敲除组细胞的RP5-919F19表达水平为0.42±0.07,相对对照组的0.89±0.08低(P<0.05)。应用CCK-8对胃癌细胞增殖能力进行检测,研究结果显示,RP5-919F19过表达组AGS细胞在培养24 h和48 h的增殖能力均相对对照组有明显提高(P<0.05),而RP5-919F19敲除组SGC-7901细胞在培养24 h和48 h的增殖能力均相对对照组低(P<0.05)。Transwell侵袭实验示RP5-919F19过表达组AGS细胞的侵袭和迁移能力均相对对照高(P<0.05),RP5-919F19敲除组SGC-7901细胞的侵袭和迁移能力均相对对照组低(P<0.05)。Western blot结果显示,相对Objective To investigate the expression of long non-coding RNA(Lnc RNA)RP5-919F19 in gastric cancer tissues and its correlation with gastric cancer invasion and metastasis.Methods Non-tumor gastric mucosa(more than 3cm away from the cancer tissue)and gastric adenocarcinoma tissues were collected from Jan.2020 to Jan.2022 in our hospital.TRIzol kit was used to extract total RNA from cells and tissues,and reverse transcription kit was used to reverse transcribed RNA into cDNA.Quantitative real-time PCR kit was used for quantitative analysis.SGC-7901 and AGS human gastric cancer cells were used to construct RP5-919F19 knockdown and overexpression models.CCK-8 assay was used to confirm cell proliferation,and Transwell invasion assay was used to confirm the invasion ability of gastric cancer cells.Results The expression of RP5-919F19 was detected in 79 cases of gastric cancer tissues and adjacent normal tissues,and it was found that the relative expression of RP5-919F19 in gastric cancer tissues was 1.51±0.05 significantly higher than that of 0.82±0.04 in adjacent normal tissues(P<0.05).The levels of RP5-919F19 in patients with different pathological conditions were compared and analyzed.The results showed that there were statistically significant differences in RP5-919F19 expression in patients with different TNM stages,distant metastasis,lymph node metastasis and different depth of invasion(P<0.05).There was no significant difference in RP5-919F19 expression among patients with different tumor sizes,ages and genders(P>0.05).AGS gastric cancer cells were transfected with RP5-919F19 overexpression plasmid and control plasmid,and the efficiency of RP5-919F19 was detected.The results showed that the expression level of RP5-919F19 in the overexpression group was 1.83±0.14 higher than that of 0.82±0.05 in the control group(P<0.05).SGC-7901 gastric cancer cells were transfected with RP5-919F19 knockout vector and control vector,and the efficiency of RP5-919F19 was detected.The results showed that the expression level o

关 键 词：RP5-919F19 胃癌 侵袭 转移 

分 类 号：R735.2[医药卫生—肿瘤]