INHBA在结直肠癌中的表达及其与微卫星状态和临床病理特征的关系  

Expression of INHBA in colorectal cancer and its relationship with microsatellite status and clinicopathological features

作　　者：马炀斐 谭琦 李琪 王亚帝 谷泽慧 李伦 陈素贤 Ma Yangfei;Tan Qi;Li Qi;Wang Yadi;Gu Zehui;Li Lun;Chen Suxian(Department of Pathology,the Third Affiliated Hospital of Jinzhou Medical University,Jinzhou 121000,China;Precision Medicine Center,the Third Affiliated Hospital of Jinzhou Medical University,Jinzhou 121000,China)

机构地区：[1]锦州医科大学附属第三医院病理科,锦州121000 [2]锦州医科大学附属第三医院精准医学中心,锦州121000

出　　处：《肿瘤研究与临床》2023年第10期733-738,共6页Cancer Research and Clinic

摘　　要：目的探讨INHBA在结直肠癌中的表达及其与微卫星状态、临床病理特征之间的关系。方法基于基因表达综合(GEO)数据库及基因表达谱交互分析(GEPIA)数据库进行生物信息学分析,选定结直肠癌差异表达预后相关目标基因。收集2016年1月至2022年6月于锦州医科大学附属第三医院行手术治疗的107例结直肠癌患者蜡块组织,制备组织芯片,采用免疫组织化学法检测结直肠癌组织中微卫星状态[错配修复(MMR)蛋白MLH1、MSH2、MSH6、PMS2均为阳性表达为错配修复完整(pMMR),代表微卫星低度不稳定或微卫星稳定;若其中任何一个指标为阴性,则判定为错配修复缺陷(dMMR),代表微卫星高度不稳定]和INHBA的表达情况。回顾性分析患者临床病理资料,分析INHBA与微卫星状态及临床病理特征之间的关系。结果从GEO数据库筛选出结直肠癌3个数据集:GSE110223(13个癌组织、13个癌旁组织)、GSE110224(17个癌组织、17个癌旁组织)、GSE113513(14个癌组织、14个癌旁组织),筛选出癌组织和癌旁组织间差异表达上调和下调的前50个基因,经韦恩图分析3个数据集的交集基因,筛选出上调基因12个,下调基因17个;根据GEPIA数据库,共有的上调和下调差异基因中AQP8、ZG16、INHBA基因与结直肠癌预后相关,INHBA在结直肠癌组织中表达水平较癌旁(距肿瘤边缘≥5 cm)组织高(P<0.05),选定INHBA基因进行分析。对收集的结直肠癌蜡块组织进行免疫组织化学检测显示,结直肠癌组织INHBA高表达患者比例高于癌旁组织INHBA高表达患者比例[85.05%(91/107)比67.29%(72/107),P<0.05]。癌组织INHBA高表达与病灶部位[右半结肠比左半结直肠:94.00%(47/50)比77.19%(44/57)]、肿瘤长径[>5 cm比≤5 cm:92.73%(51/55)比76.92%(40/52)]、浸润深度[T3~4期比T1~2期:96.43%(54/56)比72.55%(37/51)]、分化程度[低、中分化比高分化:91.04%(61/67)比75.00%(30/40)]、淋巴结转移[是比否:93.02%(40/43)比78.13%(50/64)Objective To investigate the expression of INHBA in colorectal cancer and its relationship with microsatellite status and clinicopathological features.Methods Bioinformatics analysis was conducted based on Gene Expression Omnibus(GEO)database and Gene Expression Profiling Interaction Analysis(GEPIA)database,and the differentially expressed prognosis-related target genes in colorectal cancer were selected.Wax mass tissues of 107 patients with colorectal cancer who underwent surgery from January 2016 to June 2022 in the Third Affiliated Hospital of Jinzhou Medical University were collected,and the tissue microarrays were prepared.The clinicopathological microsatellite status[positive expressions of the mismatch repair(MMR)proteins MLH1,MSH2,MSH6,and PMS2 were mismatch repair proficient(pMMR),which represented low microsatellite instability or microsatellite stabilization;if any of these indexes was negative,it was judged to be mismatch repair deficient(dMMR),which represented high microsatellite instability]and INHBA expression in colorectal cancer tissues were detected by immunohistochemistry,data of the patients were retrospectively analyzed.The relationship between INHBA and microsatellite status as well as clinicopathological features was analyzed.Results Three data sets of colorectal cancer were selected from GEO database:GSE110223(13 cancer tissues,13 paracancerous tissues),GSE110224(17 cancer tissues,17 paracancerous tissues),GSE113513(14 cancer tissues,14 paracancerous tissues),and the top 50 genes that were differentially up-regulated and down-regulated between cancer tissues and paracancerous tissues were screened.Intersection genes of 3 data sets were analyzed by Venn diagram,and 12 up-regulated genes and 17 down-regulated genes were screened out.According to GEPIA database,AQP8,ZG16 and INHBA genes among the up-regulated and down-regulated differential genes were associated with the prognosis of colorectal cancer.INHBA was higher expressed in colorectal cancer tissues than in paracancerous tissues(≥5

关 键 词：结直肠肿瘤 微卫星不稳定性 INHBA基因 

分 类 号：R735.3[医药卫生—肿瘤]