灵芝子实体中等极性三萜的制备分离  

Preparation and separation of moderate-polarity triterpenoids from basidiomata of Ganoderma lingzhi

作　　者：郭晓宇 彭小芳 周靖 王晨光 周俊 陈惠[5] 吴伟杰[5] 张劲松[1] 冯娜[1] GUO Xiaoyu;PENG Xiaofang;ZHOU Jing;WANG Chenguang;ZHOU Jun;CHEN Hui;WU Weijie;ZHANG Jingsong;FENG Na(National Engineering Research Center of Edible Fungi,National R&D Center for Processing of Edible Fungi,Key Laboratory of Resources and Utilization of Edible Fungi(South),Ministry of Agriculture and Rural Affairs,Key Laboratory of Agricultural Genetics and Breeding of Shanghai,Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Shanghai 201403,China;School of Food Science,Shanghai Ocean University,Shanghai 201306,China;Shanghai Bai Xin Bio-Tech Co.,Ltd.,Shanghai 201403,China;Anhui Huangshan Yunle Ganoderma Lucidum Co.,Ltd.,Jingde 242600,Anhui,China;Jiangsu Alphay Bio-technology Co.,Ltd.,Nantong 226009,Jiangsu,China)

机构地区：[1]上海市农业科学院食用菌研究所农业农村部南方食用菌资源利用重点实验室国家食用菌工程技术研究中心国家食用菌加工技术研发分中心上海市农业遗传育种重点开放实验室,上海201403 [2]上海海洋大学食品学院,上海201306 [3]上海百信生物科技有限公司,上海201403 [4]安徽黄山云乐灵芝有限公司,安徽旌德242600 [5]江苏安惠生物科技有限公司,江苏南通226009

出　　处：《菌物学报》2023年第12期2454-2469,共16页Mycosystema

基　　金：上海市科学技术委员会科研计划(17391903300);上海市农业科学院卓越团队建设计划[沪农科卓(2022)003]。

摘　　要：采用中压层析、中高压层析、制备薄层等多种层析方法,对灵芝子实体的中等极性三萜组分进行分离纯化,得到28个化合物。依据核磁、质谱等波谱手段对化合物结构进行解析,分别鉴定为灵芝烯酸C(1)、3β,7β,12β-trihydroxy-11,15,23-trioxo-lanost-8,20-dien-26-oic acid(2)、灵芝酸C2(3)、赤芝酸N(4)、灵芝酸C6(5)、赤芝酮B(6)、灵芝酸G(7)、灵芝酸δ(8)、灵芝烯酸B(9)、灵芝酸B(10)、灵芝烯酸H(11)、灵芝烯酸K(12)、灵芝酸LM2(13)、灵芝酸AM1(14)、灵芝酸K(15)、灵芝烯酸A(16)、赤芝酸E2(17)、灵芝烯酸E(18)、灵芝酸Df(19)、灵芝酸H(20)、灵芝酸D1(21)、灵芝烯酸D(22)、灵芝酸A(23)、灵赤酸D(24)、灵芝酸D(25)、12β-acetoxy-7β-hydroxy-3,11,15,23-tetraoxo-5α-lanosta-8,20-dien-26-oic acid(26)、灵芝烯酸F(27)和灵芝酸B甲酯(28)。该结果为灵芝子实体中含有的三萜种类调查提供了依据,也为后续的活性研究提供了较为充分的物质保障。The medium-pressure chromatography,medium-high pressure chromatography and preparative thin-layer chromatography were used to isolate and purify the moderate-polarity fractions of triterpenoids from basidiomata of Ganoderma lingzhi.The structures of the compounds were identified on the basis of NMR and mass spectrometry.The following 28 compounds were obtained:ganoderenic acid C(1),3β,7β,12β-trihydroxy-11,15,23-trioxolanost-8,20-dien-26-oic acid(2),ganoderic acid C2(3),lucidenic acid N(4),ganoderic acid C6(5),lucidone B(6),ganoderic acid G(7),ganoderic acidδ(8),ganoderenic acid B(9),ganoderic acid B(10),ganoderenic acid H(11),ganoderenic acid K(12),ganoderic acid LM2(13),ganoderic acid AM1(14),ganoderic acid K(15),ganoderenic acid A(16),lucidenic acid E2(17),ganoderenic acid E(18),ganoderic acid Df(19),ganoderic acid H(20),ganoderic acid D1(21),ganoderenic acid D(22),ganoderic acid A(23),ganolucidic acid D(24),ganoderic acid D(25),12β-acetoxy-7β-hydroxy-3,11,15,23-tetraoxo-5α-lanosta-8,20-dien-26-oic acid(26),ganoderenic acid F(27),and methyl ganoderic acid B(28).The results afford adequate materials for subsequent bio-activity studies of the compounds.

关 键 词：灵芝子实体 三萜 分离纯化 结构鉴定 

分 类 号：TS219[轻工技术与工程—粮食、油脂及植物蛋白工程]