基于高分辨质谱的食品致敏蛋白检测研究进展  

Research Progress on Detection of Food Allergic Protein Based on High-resolution Mass Spectrometry

作　　者：刘楠 许利丽 张晓梅 宗爱珍 徐同成 尤艳莉[1] 张鸿伟 肖晶 LIU Nan;XU Li-li;ZHANG Xiao-mei;ZONG Ai-zhen;XU Tong-cheng;YOU Yan-li;ZHANG Hong-wei;XIAO Jing(College of Life Sciences,Yantai University,Yantai 264000,China;Institute of Agricultural Products Processing and Nutrition,Shandong Academy of Agricultural Sciences,Jinan 250000,China;Technology Center of Qingdao Customs District,Qingdao 266100,China;China National Center for Food Safety Risk Assessment,Beijing 100022,China)

机构地区：[1]烟台大学生命科学学院,山东烟台264000 [2]山东省农业科学研究院农产品加工与营养研究所,山东济南250000 [3]青岛海关技术研究中心,山东青岛266100 [4]国家食品安全风险评估中心,北京100022

出　　处：《分析测试学报》2023年第12期1666-1672,共7页Journal of Instrumental Analysis

基　　金：山东省重点研发计划(重大科技创新工程)项目(2021CXGC010807);青岛海关科研项目(QK202101)。

摘　　要：食品致敏原事关食品安全,其检测技术的进步对于食物过敏风险评估具有重要现实价值。与传统的酶联免疫吸附(ELISA)等免疫学检测方法相比,高分辨质谱技术(HRMS)凭借高通量等特点,近些年在食品致敏蛋白检测研究中的应用日渐广泛。该文主要综述了基于HRMS技术的致敏蛋白检测研究进展,重点介绍了致敏蛋白的提取纯化、酶解以及基于HRMS的分析策略和数据采集模式,同时分析了HRMS在致敏蛋白检测方面存在的挑战,以期为促进致敏蛋白检测的现代化发展提供参考。Food allergen is tightly related to food safety,and technological progresses on the issue are realistically valuable to food allergy risk assessment.Compared with conventional immunological technique such as enzyme-linked immunosorbent assay(ELISA),high-resolution mass spectrometry(HRMS)is burgeoning in recent years for food allergic protein with its high-throughput characteriza⁃tion.In this paper,the research progress of HRMS technology for allergic protein detection is mainly reviewed,and extraction,purification and enzymatic hydrolysis of allergic protein,as well as HRMS-based analysis strategy and data acquisition is emphatically highlighted.Additionally,the challenges in the detection of allergic protein were also analyzed.

关 键 词：高分辨质谱 致敏蛋白 检测 

分 类 号：O657.6[理学—分析化学] F407.82[理学—化学]