海南产温郁金悬浮细胞培养及其挥发性成分的积累研究  

Study on Suspension Cell Culture and Volatile Components Accumulation of Curcuma wenyujin in Hainan

作　　者：董丽霞 陈健妙[1,2] 曾劲松 于靖[1] 黄娴[3] DONG Li-xia;CHEN Jian-miao;ZENG Jin-song;YU Jing;HUANG Xian(Sanya Nanfan Research Institute of Hainan University,Sanya 572024,China;College of Tropical Crops,Hainan University/Key Laboratory of Sustainable Utilization of Tropical Biological Resources in Hainan Province,Haikou 570228,China;Hainan Bikai Pharmaceutical Co.,Ltd.,Haikou 570216,China)

机构地区：[1]海南大学三亚南繁研究院,海南三亚572024 [2]海南大学热带作物学院/海南省热带生物资源可持续利用重点实验室,海南海口570228 [3]海南碧凯药业有限公司,海南海口570216

出　　处：《中药材》2023年第4期817-822,共6页Journal of Chinese Medicinal Materials

基　　金：海南省高等学校教育教学改革研究项目(No.Hnjg2019-3)。

摘　　要：目的:建立海南地区引种温郁金Curcuma wenyujin的悬浮细胞系,并初步研究离体培养材料中挥发性成分的积累。方法:甄选诱导愈伤组织的外植体、培养基以及悬浮细胞起始愈伤组织等,建立稳定的悬浮细胞系;采用顶空固相微萃取-气质联用技术测定温郁金悬浮细胞团等离体培养材料中莪术挥发油等挥发性成分。结果:嫩根诱导的愈伤组织是建立稳定悬浮细胞系的适宜起始材料;愈伤组织继代培养2次后增殖较明显,可形成颜色鲜黄、颗粒均一、疏松易碎的胚性愈伤组织;三种离体培养材料中,常见挥发性物质总量检出率依次为组培苗、悬浮细胞团、愈伤组织;莪术挥发油中常见化学成分牻牛儿酮、莪术烯、莪术二酮、榄香烯等总量的检出率依次为组培苗、愈伤组织、悬浮细胞团。结论:海南地区引种温郁金悬浮细胞系等离体培养材料均积累了一定量的莪术油等挥发性有效成分,为进一步筛选优化培养体系、促进培养物次生代谢物的积累提供了基础研究。Objective:To establish a suspension cell line ofCurcuma wenyujinintroduced from Hainan,and to study the accumula⁃tion of volatile components in culture materialsin vitro.Methods:The callus⁃inducing explants,culture⁃medium and suspension cell initi⁃ation callus were selected to establish stable suspension cell lines.The volatile components in culture materialsin vitrosuch as suspen⁃ded cell mass ofCurcuma wenyujinwere determined by headspace solid phase microextraction⁃gas chromatography⁃Mass spectrometry.Results:The callus induced by young roots was a suitable starting material for the establishment of stable suspension cell lines.After secondary culture for two times,the callus proliferated obviously,and it was easy to form embryogenic callus with bright yellow color,u⁃niform granules,loose and fragile.Among the three culture materialsin vitro,the detection rates of total volatile substances were tissue culture seedlings,suspended cell mass,callus in order.The detection rates of common chemical ingredients ofCurcuma wenyujinvolatile oil,including germacrone,curzerene,curdione,elemene,were tissue culture seedlings,callus,suspended cell mass in order.Conclusion:A certain amount of volatile active components such as zedoary oil are accumulated in the culture materialsin vitrosuch as the suspen⁃ded cell line ofCurcuma wenyujinintroduced in Hainan,which provides basic research for further screening and optimization of culture system and promoting the accumulation of secondary metabolites in culture.

关 键 词：海南引种温郁金 愈伤组织 悬浮细胞系 挥发性成分 

分 类 号：R282.2[医药卫生—中药学]