湖南产山橿根中一个新吡喃酮  

A New Pyrone from the Roots of Lindera reflexa in Hunan Province

作　　者：韩月 孙孝亚 付宇航 田志浩 段懿哲 陈随清 HAN Yue;SUN Xiao-ya;FU Yu-hang;TIAN Zhi-hao;DUAN Yi-zhe;CHEN Sui-qing(College of Pharmacy,Henan University of Chinese Medicine/Henan Key Laboratory of Chinese Medicine Resources and Chinese Medicine Chemistry,Zhengzhou 450046,China;Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases co-constructed by Henan Province&Education Ministry of P.R.China,Zhengzhou 450046,China)

机构地区：[1]河南中医药大学药学院/河南省中药资源与中药化学重点实验室,河南郑州450046 [2]呼吸疾病中医药防治省部共建协同创新中心,河南郑州450046

出　　处：《中药材》2023年第4期878-881,共4页Journal of Chinese Medicinal Materials

基　　金：河南省科技攻关项目(212102310353);国家自然科学基金项目(81773859)。

摘　　要：目的:研究湖南产山橿根70%乙醇提取物的乙酸乙酯部位化学成分及其体外抗炎作用。方法:利用MCI、硅胶和半制备高效液相等多种色谱技术对山橿根的化学成分进行分离纯化,通过化合物的理化性质及核磁数据鉴定结构;以脂多糖(LPS)诱导的RAW 264.7巨噬细胞作为炎症模型,探究化合物对炎症因子NO、IL-6、TNF-α释放量的影响。结果:从湖南地区山橿根70%乙醇提取物的乙酸乙酯部位中分离鉴定了6个吡喃酮类化合物,分别为:(E)-5-(hydroxymethyl)-4-methoxy-6-styryl-2H-pyran-2-one(1)、5,6-dehydrokavain(2)、4-methoxy-6-(2-hydroxy-4-phenylbutyl)-2H-pyran-2-one(3)、4-methoxy-6-(11,12-dimethoxy-trans-styryl)-2-pyrone(4)、rel-,trans-3-bis-[6-(4-methoxy-2-pyronyl)]-cis-2,trans-4-diphenyl cyclobutane(5)、aniba dimer A(6)。体外抗炎实验表明,化合物1和4能有效抑制炎症因子NO、IL-6、TNF-α的释放量。结论:其中,化合物1为新化合物,化合物2~6为首次从山橿中分离得到。化合物1和4有良好的抗炎作用。Objective:To study the chemical constituents of ethyl acetate extract from the 70%ethanol extract ofLindera reflexa roots and the anti⁃inflammatory effectsin vitro.Methods:The compounds from roots ofLindera reflexawere isolated and purified by vari⁃ous chromatographic techniques,such as MCI,silica gel and semi⁃preparative liquid phase.The structures were identified by physico⁃chemical properties and NMR data.The lipopolysaccharide(LPS)induced RAW 264.7 macrophages were used as the inflammatory model to investigate the effects of the compounds on the release of inflammatory cytokines NO,IL⁃6 and TNF⁃α.Results:Six pyrones were isolated from ethyl acetate extract of 70%ethanol extract ofLindera reflexaroots and identified as(E)⁃5⁃(hydroxymethyl)⁃4⁃me⁃thoxy⁃6⁃styryl⁃2H⁃pyran⁃2⁃one(1),5,6⁃dehydrokavain(2),4⁃methoxy⁃6⁃(2⁃hydroxy⁃4⁃phenylbutyl)⁃2H⁃pyran⁃2⁃one(3),4⁃methoxy⁃6⁃(11,12⁃dimethoxy⁃trans⁃styryl)⁃2⁃pyrone(4),rel⁃,trans⁃3⁃bis⁃[6⁃(4⁃methoxy⁃2⁃pyronyl)]⁃cis⁃2,trans⁃4⁃diphenyl cyclobutane(5),aniba dimer A(6),respectively.In vitroanti⁃inflammatory experiments showed that compounds 1,4 could effectively inhibit the release of inflammatory factors NO,IL⁃6 and TNF⁃α.Conclusion:Among them,compound 1 is a new compound,compounds 2 to 6 are isolated fromLindera reflexafor the first time.Compounds 1,4 have great anti⁃inflammatory effects.

关 键 词：山橿 化学成分 体外抗炎 吡喃酮 

分 类 号：R284.1[医药卫生—中药学] R284.2[医药卫生—中医学]