超高效液相色谱法同时测定预混剂中6种促生长类抗菌药物的研究  

Study on simultaneous determination of six kinds of growth promoting antibacterial drugs in premixes by ultra-high performance liquid chromatography

作　　者：戴青[1] 顾进华[1] 张秀英[1] 赵晖[1] 杨秀玉[1] 王轩 韩宁宁[1] DAI Qing;GU Jin-hua;ZHANG Xiu-ying;ZHAO Hui;YANG Xiu-yu;WANG Xuan;HAN Ning-ning

机构地区：[1]中国兽医药品监察所,北京100081

出　　处：《饲料研究》2023年第24期112-116,共5页Feed Research

基　　金：“十四五”国家重点研发计划项目(项目编号:2022YFD1801105);兽药行业公益性重点专项项目(项目编号:GY202001)。

摘　　要：试验旨在建立超高效液相色谱法同时测定预混剂中6种促生长类抗菌药物的检测方法。以乙腈-0.25%甲酸水溶液(50∶50)作为溶剂,采用超声提取法对样品进行前处理,以0.05 mol/L甲酸铵水溶液(用甲酸调节pH值至3.8)-乙腈作为流动相,采用梯度淋洗和C18色谱柱进行分离。结果显示,6种抗菌药物的检测限为1~10 mg/g,在制剂添加浓度为2~100 mg/g的范围内,利用试验所用方法检测得到的6种促生长类抗菌药物主成分的峰面积与制剂添加浓度呈良好的线性关系,相关系数R2为0.9994~1.0000,平均回收率为92.2%~104.6%。研究表明,试验所用方法快速简便、准确可信、灵敏度高,适合预混剂中促生长类抗菌药物的检测。The study was to establish a method for the simultaneous determination of six growth promoting antibacterial agents in premixes by ultra-high performance liquid chromatography.An acetonitrile-0.25%formic acid aqueous solution(50∶50)was used as the solvent,and the samples were pretreated by ultrasonic extraction.A 0.05 mol/L ammonium formate aqueous solution(pH value adjusted to 3.8 with formic acid)-acetonitrile was used as the mobile phase,and the samples were separated by gradient elution and C18 column.The results showed that the detection limit of six antibacterial agents was 1~10 mg/g.In the range of 2~100 mg/g of the added concentration,the peak areas of the main components of the six growth promoting antibacterial agents detected by the method had a good linear relationship,and the correlation coefficient R2 was 0.9994~1.0000.The average recoveries of six antibacterial agents were 92.2%~104.6%.The study indicates that the proposed method is efficient,reliable and sensitive,which can be used for the detection of growth promoting antibacterial agents in premixes.

关 键 词：超高效液相色谱法 预混剂 促生长类抗菌药物 

分 类 号：S816.17[农业科学—饲料科学]