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作 者:王成金 吕琳 张玉琳 熊忆茗 陈宣樾 秦占芬[2] 王燕飞 林军 Wang Chengjin;Lv Lin;Zhang Yulin;Xiong Yiming;Chen Xuanyue;Qin Zhanfen;Wang Yanfei;Lin Jun(Inner Mongolia Technical Center of Solid Waste and Soil Ecology,Huhhot 010011,China;State Key Laboratory of Environmental Chemistry and Ecotoxicology,Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085,China;Solid Waste and Chemicals Management Center,Ministry of Ecology and Environment,Beijing 100029,China)
机构地区:[1]内蒙古自治区固体废物与土壤生态环境技术中心,呼和浩特010011 [2]中国科学院生态环境研究中心,环境化学与生态毒理学国家重点实验室,北京100085 [3]生态环境部固体废物与化学品管理技术中心,北京100029
出 处:《生态毒理学报》2023年第6期60-67,共8页Asian Journal of Ecotoxicology
基 金:国家自然科学基金资助项目(2207060550)。
摘 要:基于哺乳动物的传统毒性测试方法存在周期长、成本高、通量低、有动物伦理限制等诸多问题。斑马鱼因为个体小、发育快、易于饲养等特点,适用于构建基于多孔板的中高通量毒性测试方法。本文对基于多孔板的斑马鱼急性毒性、发育毒性、行为和微行为分析等几个中高通量测试方法进行了综述,分析了各自的优缺点及应用现状。另外介绍了高通量测试在自动化操作方面的最近进展。最后分析了斑马鱼中高通量毒性测试方法未来的发展趋势和应用前景,提出应着重开展对现有方法的验证和优化工作,减少对人工的依赖,实现自动化操作和标准化分析,同时开发可更敏感检出发育毒性的分子或者信号通路的测试指标。随着斑马鱼高通量毒性测试方法不断的发展和优化,其在化学物质毒性筛查和风险评估中将发挥越来越重要的作用。Traditional mammalian-based toxicity testing methods have many drawbacks such as long test duration,high cost,low throughput and animal ethical restrictions.Zebrafish is suitable for the construction of high-throughput toxicity testing methods due to its outstanding features,such as being smaller in size,rapid growth and easy to feed.In this paper,existing middle and high throughput testing methods for analyzing the acute toxicity,development toxicity and behaviors of zebrafish based on porous plates were reviewed,and their advantages,disadvantages and application status were analyzed.Recent research progress on automatic operations for high-throughput testing was also introduced.The development trend and application prospects of middle/high-throughput toxicity testing methods using zebrafish were discussed as well.It is believed that the validation and refinement of existing m ethods should be emphasized to improve automation and standardization,Meanwhile,more refined molecular or signaling pathway endpoints for sensitive detection of developmental toxicity should be developed.It is foreseeable that zebrafish-based middle/high-throughput testing methods will play significant roles in toxicity screening and risk assessment of chemicals.
分 类 号:X171.5[环境科学与工程—环境科学]
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