LncRNA DSCAM-AS1调节miR-150-5p/BRAF轴对甲状腺癌细胞恶性生物学行为的影响  

Impact of LncRNA DSCAM⁃AS1 on the malignant biological behaviors of thyroid cancer cells by regulat⁃ing the miR⁃150⁃5p/BRAF axis

作　　者：彭云[1] 温美玲 吕云霞[2] 陈万志[2] 何春[1] 俞建平 丁振罗 PENG Yun;WEN Meiling;LV Yunxia;CHEN Wanzhi;HE Chun;YU Jian-ping;DING Zhenluo(Department of Thyroid Surgery,People's Hospital of Ganzhou,Ganzhou 341000,China)

机构地区：[1]赣州市人民医院甲状腺外科,江西赣州341000 [2]南昌大学第二附属医院甲状腺外科,南昌330000

出　　处：《实用医学杂志》2023年第23期3043-3050,共8页The Journal of Practical Medicine

基　　金：国家自然科学基金项目(编号:82204879);江西省卫生健康委员会科技计划项目(编号:202311874);江西省卫生健康委员会科技计划项目(编号:20194051)。

摘　　要：目的探讨lncRNA DSCAM-AS1调节miR-150-5p/BRAF轴对甲状腺癌(TC)细胞恶性生物学行为的影响。方法qRT-PCR检测TC细胞中DSCAM-AS1表达;FISH、pull down实验、双荧光素酶报告基因实验验证DSCAM-AS1、BRAF与miR-150-5p靶向调控关系;检测SW579细胞增殖、迁移和侵袭,Western blot检测BRAF、E-cadherin、vimentin蛋白表达;小鼠体内肿瘤形成实验验证DSCAM-AS1对TC肿瘤生长的影响。结果DSCAM-AS1在TC细胞中高表达(P<0.05);DSCAM-AS1、BRAF与miR-150-5p存在靶向调控关系;抑制DSCAM-AS1表达或过表达miR-150-5p可显著抑制SW579细胞增殖、迁移、侵袭及EMT(P<0.05);抑制miR-150-5p表达或过表达BRAF可逆转抑制DSCAM-AS1表达或过表达miR-150-5p对SW579细胞恶性行为的抑制作用(P<0.05);体内实验显示,抑制DSCAM-AS1表达可显著抑制小鼠移植瘤的生长(P<0.05)。结论DSCAM-AS1在TC细胞中上调表达,抑制DSCAM-AS1表达可通过调节miR-150-5p/BRAF信号轴,抑制TC恶性进展。Objective To investigate the impact of lncRNA DSCAM-AS1 on the malignant biological behaviors of thyroid cancer(TC)cells by regulating the miR-150-5p/BRAF axis.Methods The expression of DSCAM-AS1 in TC cells was detected by qRT-PCR,and the best intervention cell line was screened.;the relationship between DSCAM-AS1,BRAF and miR-150-5p targeting regulation was verified by FISH,pull down and double Luciferase reporter gene experiment;The proliferation,migration and invasion of SW579 cells were detected;Western blot was applied to detect the expression of BRAF,E-Cadherin,and vimentin proteins;the tumor formation experiment in mice was applied to verify the effect of DSCAM-AS1 on TC tumor growth.Results DSCAM-AS1 was highly expressed in TC tissue and cells(P<0.05);There is a targeted regulation relationship between DSCAM-AS1,BRAF and miR-150-5p;inhibition of DSCAM-AS1 expression or overexpression of miR-150-5p obviously inhibited the proliferation,migration,invasion,and EMT of SW579 cells(P<0.05);Inhibition of miR150-5p expression or overexpression of BRAF reversed the inhibitory effect of inhibition of DSCAM-AS1 expression or overexpression of miR-150-5p on the malignant behavior of SW579 cells(P<0.05);in vivo experiments showed that inhibiting the expression of DSCAM-AS1 obviously inhibited the growth of transplanted tumors in mice(P<0.05).Conclusion DSCAM-AS1 is up-regulated in TC cells,inhibiting the expression of LncRNA DSCAMAS1 can inhibit the malignant progression of TC by regulating the miR-150-5p/BRAF signaling axis.

关 键 词：lncRNA DSCAM-AS1 miR-150-5p/BRAF轴 甲状腺癌 增殖 迁移 侵袭 

分 类 号：R736.1[医药卫生—肿瘤]