基于Top-down技术评定液相色谱法测定棉籽油中游离棉酚的不确定度  

Evaluation of Uncertainty of Determination of Free Gossypol in Cottonseed Oil by Liquid Chromatography Based on Top-Down Technique

作　　者：孙晓晗 李烁 SUN Xiaohan;LI Shuo(Jinan Inspection and Testing Center for Food and Drug Control,Jinan 250102,China)

机构地区：[1]济南市食品药品检验检测中心,山东济南250102

出　　处：《食品安全导刊》2023年第33期121-123,136,共4页China Food Safety Magazine

摘　　要：目的:利用Top-down技术评定液相色谱法测定棉籽油中游离棉酚的不确定度。方法:按照《食品安全国家标准植物性食品中游离棉酚的测定》(GB 5009.148—2014)规定的分析方法,在期间精密度条件下,对棉籽油质控样品中游离棉酚的含量进行测定,基于Top-down法的原理对检测数据进行分析。结果:在证明分析结果呈正态性、独立性且偏倚受控的情况下,不确定度为1.56 mg·kg^(-1)。结论:Top-down技术将不确定度评定与实验室内部质量控制工作有机结合起来,客观地监测实验室的期间精密度水平,在化学分析领域具有很强的可操作性。Objective:To evaluate the uncertainty of determination of free gossypol in cottonseed oil by liquid chromatography with Top-down technique.Method:According to the analysis method specified in GB 5009.148—2014,the content of free gossyol in the quality control sample of cottonseed oil was determined under the condition of period precision,and the detection data was analyzed based on the principle of Top-down method.Result:An uncertainty of 1.56 mg·kg^(-1) was assessed with normality,independence,and controlled bias demonstrated.Conclusion:The Top-down technique combines the uncertainty assessment with the internal quality control of the laboratory,objectively monitors the period precision level of the laboratory,and has strong operability in the field of chemical analysis.

关 键 词：游离棉酚 不确定度 液相色谱法 

分 类 号：O657.72[理学—分析化学] TS225.1[理学—化学]