环磷酰胺聚乳酸微球的制备及其缓释过程的研究  

Preparation of Cyclophosphamide Polylactic Acid Microspheres and Study on Their Sustained Release Process

作　　者：吴慧敏 钱文斌 WU Hui-min;QIAN Wen-bin(School of Pharmacy,Xianning Medical College,Hubei University of Science and Technology,Xianning Hubei 437100,China)

机构地区：[1]湖北科技学院医学部药学院,湖北咸宁437100 [2]湖北科技学院医学部基础医学院

出　　处：《湖北科技学院学报（医学版）》2023年第6期486-490,505,共6页Journal of Hubei University of Science and Technology(Medical Sciences)

基　　金：校国家级科研项目培育计划项目(2021-23GP03)。

摘　　要：目的制备环磷酰胺聚乳酸微球,并检验微球的体外缓释效果。方法采用O/W型乳化溶剂挥发法制备载药微球,通过单因素实验优化制备工艺,用生物显微镜观察微球的形态,并用激光粒度分析仪分析粒径。用紫外-可见光谱法研究环磷酰胺聚乳酸微球的载药量、包封率和体外释药性能。结果微球呈球形,直径为(12.67±3.67)μm。载药量和包封率分别为15.38%和62.5%,累计释放时间为108h,累计释放率为84.07%。结论本研究成功制备环磷酰胺聚乳酸微球,为制备可生物降解载药微球提供参考。Objective To prepare cyclophosphamide polylactic acid microspheres and test the sustained release effect of microspheres in vitro.Methods Drug-loaded microspheres were prepared by O/W emulsion solvent evaporation method,and the preparation process was optimized by single factor experiment.The morphology of microspheres was observed by biomicroscope,and the particle size was analyzed by laser particle size analyzer.The drug loading,encapsulation efficiency and drug release in vitro of cyclophosphamide polylactic acid microspheres were studied by ultraviolet-visible spectrometry.Results The microspheres were spherical with a diameter of(12.67±3.67)μm.The drug loading and encapsulation rates were 15.38%and 62.5%,respectively,with a cumulative release time of 108 h and a cumulative release rate of 84.07%.Conclusion Cyclophosphamide polylactic acid microspheres were successfully prepared in this study,which provided a reference for the preparation of biodegradable drug-loaded microspheres.

关 键 词：环磷酰胺 聚乳酸 微球 体外模拟 

分 类 号：R94[医药卫生—药剂学]