BSA-Seq结合RNA-Seq技术挖掘大豆叶片提前黄化衰老基因  

Mine the genes of premature yellowing and aging in soybean leaves by BSA-seq combined with RNA-seq technology

作　　者：李世宽 洪慧龙[2] 付佳祺 谷勇哲 孙如建 邱丽娟 LI Shi-Kuan;HONG Hui-Long;FU Jia-Qi;GU Yong-Zhe;SUN Ru-Jian;QIU Li-Juan(College of Life Sciences and Technology,Harbin Normal University,Harbin 150025,Heilongjiang,China;Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China;Hulun Buir Institution of Agricultural Sciences,Zhalantun 162650,Inner Mongolia,China)

机构地区：[1]哈尔滨师范大学生命科学与技术学院,黑龙江哈尔滨150025 [2]中国农业科学院作物科学研究所,北京100081 [3]呼伦贝尔市农业科学研究所,内蒙古扎兰屯162650

出　　处：《作物学报》2024年第2期294-309,共16页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(32172005)资助。

摘　　要：大豆产量与其生殖生长的持续时间呈正相关,延缓其开花后叶片的衰老,提高其生理功能,有利于增加植物的粒重。叶片黄化是植物衰老的显著特征之一。关于在大豆鼓粒后期叶片黄化的研究鲜见报道。本研究对鼓粒后期叶片提前黄化突变体ly和野生型ofc杂交组合进行遗传分析,结果表明,大豆鼓粒后期叶片提前黄化性状受单隐性核基因控制。通过BSA-Seq在19号染色体得到一个2.23Mb的初定位区间,经开发标记图位克隆后将区间缩短至1.75 Mb,区间内有219个基因,再结合RNA-Seq分析,得到了区间内12个候选基因,其中有4个SNP变异基因和8个差异表达基因。本研究结果为大豆鼓粒后期叶片黄化衰老基因的克隆奠定了基础。The yield of soybean is positively correlated with the duration of reproductive growth,which delays the aging of leaves after flowering,enhances their physiological performance,and supports growing plants with heavier grains.Leaf yellowing is one of the distinctive features of plant aging.Studies on leaf yellowing at the late stage of soybean drum grain have rarely been reported.The early yellowing feature of soybean late tympanic leaves was controlled by a single recessive nuclear gene,according to the genetic analysis of the hybrid of the early yellowing mutant ly and wild-type ofc in this study.Using Molecular Marker to Map-Based Cloning,a 2.23 Mb preliminary localization interval on chromosome 19 was obtained.The interval was shortened to 1.75 Mb and contained 219 genes.When this interval was combined with RNA-Seq analysis,12 candidate genes were detected,including 4 SNP variant genes and 8 differentially expressed genes.The findings of this study provides the framework for the cloning of genes that cause aging and yellowing during the filling later period in soybean.

关 键 词：大豆 突变体 BSA-Seq RNA-SEQ 图位克隆 

分 类 号：S565.1[农业科学—作物学]