ECT2在宫颈癌组织中的表达及对预后的影响  

Expression of ECT2 in cervical cancer and its effect on the prognosis of cervical cancer

作　　者：陈宇[1,2] 宋紫烨 高阳 蔡红兵 CHEN Yu;SONG Ziye;GAO Yang;CAI Hongbing(Xiaogan Hospital Affiliated to Wuhan University of Science and Technology&The Central Hospital of Xiaogan,Xiaogan 432100,Hubei,China;Dept.of Gynecological Oncology,Zhongnan Hospital of Wuhan University,Hubei Cancer Clinical Study Center,&Hubei Key Laboratory of Tumor Biological Behaviors,Wuhan 430071,Hubei,China;Erasmus University Rotterdam,Rotterdam 999025,Netherlands)

机构地区：[1]武汉科技大学附属孝感医院/孝感市中心医院,湖北孝感432100 [2]武汉大学中南医院妇瘤科/湖北省肿瘤医学研究中心/肿瘤生物学行为湖北省重点实验室,湖北武汉430071 [3]鹿特丹伊拉斯姆斯大学,荷兰鹿特丹999025

出　　处：《武汉大学学报（医学版）》2023年第12期1456-1460,共5页Medical Journal of Wuhan University

基　　金：国家自然科学基金资助项目(编号:81972447)。

摘　　要：目的:了解上皮细胞转化序列2(ECT2)在宫颈癌组织与癌旁组织中的表达水平差异及其临床意义。方法:利用Oncomine数据库分析ECT2在宫颈癌组织和癌旁组织中的表达水平,GEPIA软件分析ECT2表达水平与预后的关系。免疫组织化学染色分析ECT2在宫颈癌组织和癌旁组织中的蛋白表达水平及其与临床病理特征的关系。构建ECT2过表达的Hela细胞和ECT2敲降的Siha和C33a细胞。划痕实验检测细胞迁移能力。结果:与癌旁组织相比,宫颈癌组织的ECT2的mRNA及蛋白表达水平显著升高(P<0.001);ECT2与宫颈癌患者病理分级相关(P<0.05),且ECT2高表达的宫颈癌患者预后更差;ECT2促进宫颈癌细胞迁移。结论:ECT2在宫颈癌组织中表达水平较癌旁组织更高,通过促进宫颈癌细胞迁移而影响宫颈癌患者的预后。Objective:To study the expression of epithelial cell transformation sequence 2(ECT2)in cervi-cal cancer patients and its clinical significance.Methods:The Oncomine database was used to analyze the expression of ECT2 in cervical cancer patients,and the GEPIA was used to analyze its prognostic value.Immunohistochemical staining was used to analyze the protein expression of ECT2 in cervical cancer and paracancerous tissues and its relationship with clinicopathological characteristics.Then we constructed ECT2 overexpressed cervical cancer cell Hela(Hela-ECT2)and ECT2 knocked down cer-vical cancer cells Siha(Siha-siRNA)and C33a(C33a-siRNA).Scratch test was adopted to detect cellmigration ability.Results:The mRNA and protein levels of ECT2 in patients with cervical cancer were significantly increased(P<0.001),and ECT2 was related to the pathological grade of cervical cancer patients(P<0.05).The cervical cancer patients with high expression of ECT2 had a worse prognosis,and ECT2 promoted cervical cancer cells'migration.Conclusion:Epithelial cell transfor-mation sequence 2 is highly expressed in cervical cancer,and affects the prognosis of cervical cancer by promoting the migration of cervical cancer cells.

关 键 词：宫颈癌 上皮细胞转化序列2 迁移 预后 

分 类 号：R737.31[医药卫生—肿瘤]