十溴联苯醚与镉复合污染对水稻种子萌发与幼苗生长的毒性效应  

Combined effects of decabromodiphenyl ether and cadmium on the seed germination and seedling growth of rice(Oryza sativa L.)

作　　者：卢云青 周长瑞 杨垚 彭程 林匡飞[1] LU Yunqing;ZHOU Changrui;YANG Yao;PENG Cheng;LIN Kuangfei(School of Resource and Environmental Engineering,East China University of Science and Technology,Shanghai 200237,China;School of Municipal and Environmental Engineering,Henan University of Urban Construction,Pingdingshan 467036,China;Shanghai Institute of Pollution Control and Ecological Security,Shanghai 200092,China)

机构地区：[1]华东理工大学资源与环境工程学院,上海200237 [2]河南城建学院市政与环境工程学院,河南平顶山467036 [3]上海污染控制与生态安全研究院,上海200092

出　　处：《农业环境科学学报》2023年第12期2752-2762,共11页Journal of Agro-Environment Science

基　　金：国家重点研发计划项目(2019YFC1803701);国家自然科学基金项目(42177395)。

摘　　要：为评估十溴联苯醚(BDE-209)与镉(Cd)的联合植物毒性,本研究通过水培试验研究了Cd和BDE-209单独及联合作用对水稻种子发芽、幼苗生长、光合色素含量和抗氧化酶活性的影响。结果表明:单独作用下,低浓度Cd和BDE-209对种子发芽和生长均表现为促进作用,而高浓度胁迫则会导致各项指标的降低;两种污染物的联合作用在低浓度Cd胁迫下(1 mg·L^(-1))主要表现为协同效应,在高浓度Cd处理中(50 mg·L^(-1))表现为拮抗作用。Cd与各项生长指标之间均存在显著的剂量-效应关系,其中根部对Cd胁迫的响应最敏感,而BDE-209仅与根鲜质量和根伸长间存在剂量-效应关系。Cd和BDE-209单独胁迫下水稻种子根鲜质量的半数抑制浓度分别为5.05 mg·L^(-1)和6.09 mg·L^(-1)。Cd和BDE-209均能提高水稻幼苗体内的抗氧化酶活性,过氧化物酶和超氧化物歧化酶分别对Cd污染和BDE-209污染有良好的指示作用。研究表明,相较于Cd的单独污染,BDE-209的存在能够缓解高浓度Cd对种子萌发和幼苗生长的毒害作用。In order to evaluate the combined phytotoxicity of decabromodiphenyl ether(BDE-209)and cadmium(Cd),hydroponic experiments were conducted;we specifically evaluated the singular and combined effects of BDE-209 and Cd on the seed germination,seedling upgrowth,photosynthetic pigments,and antioxidant enzymes activities of Oryza sativa L.The results of this study showed that the singular effect of Cd and BDE-209 at low concentrations was the promotion of germination and growth of seeds,whereas higher Cd-or BDE-209-induced stress led to a corresponding decrease in various parameters.The combined effect of BDE-209 and Cd was synergistic at 1 mg·L^(-1)of Cd-induced stress and antagonistic at 50 mg·L^(-1)of Cd-induced stress.There was a significant dose-response relationship between Cd and the growth parameters of O.sativa,among which the root was the most sensitive.Further,there was a dose-response relationship between BDE-209 and root parameters.The calculated IC50 values for root biomass were 5.05 mg·L^(-1)and 6.09 mg·L^(-1)under Cd-induced stress and BDE-209-induced stress,respectively.The singular effect of Cd and BDE-209 treatment was the enhancement of antioxidant enzyme activities in rice seedling leaves.Specifically,SOD was determined to act as an effective biomarker for BDE-209 contamination,while POD was more sensitive to Cd contamination.Overall,this study demonstrated that BDE-209 could alleviate the toxic effects of highconcentration Cd on seed germination and seedling growth.

关 键 词：十溴联苯醚 镉 复合污染 发芽 植物毒性 水稻 

分 类 号：X173[环境科学与工程—环境科学]