环状RNA hsa_circ_0085576调控微小RNA-498/B细胞特异性莫洛尼鼠白血病病毒整合位点1轴对口腔鳞状细胞癌细胞迁移和侵袭的影响  

Effects of circular RNA hsa_circ_0085576 on cell migration and invasion of oral squamous cell carcinoma by regulating the microRNA-498/B-cell-specific Moloney murine leukemia virus integration site 1 axis

作　　者：李立恒[1] 王蕊[1] 王晓明[1] 张智轶 张璇 安峰[1] 王芹[1] 张凡[2] Li Liheng;Wang Rui;Wang Xiaoming;Zhang Zhiyi;Zhang Xuan;An Feng;Wang Qin;Zhang Fan(Dept.of Stomatology,the First Affiliated Hospital of Hebei North University,Zhangjiakou 075000,China;Dept.of Pathology,the First Affiliated Hospital of Hebei North University,Zhangjiakou 075000,China)

机构地区：[1]河北北方学院附属第一医院口腔科,张家口075000 [2]河北北方学院附属第一医院病理科,张家口075000

出　　处：《国际口腔医学杂志》2024年第1期60-67,共8页International Journal of Stomatology

基　　金：2021年度河北省医学科学研究课题计划(20210802)。

摘　　要：目的探究环状RNA hsa_circ_0085576对口腔鳞状细胞癌(OSCC)细胞迁移和侵袭的影响及其分子机制。方法使用实时荧光定量聚合酶链反应(qRT-PCR)和蛋白印迹法检测OSCC细胞中hsa_circ_0085576、微小RNA-498(miR-498)以及B细胞特异性莫洛尼鼠白血病病毒整合位点1(BMI-1)的表达水平。使用CCK-8、划痕实验、Transwell实验以及qRT-PCR、蛋白印迹法分别检测SCC-15细胞增殖活力、迁移及侵袭能力以及相关基因和蛋白的相对表达量。结果OSCC细胞中hsa_circ_0085576与BMI-1表达上调,miR-498表达下调(P<0.05)。下调hsa_circ_0085576表达或过表达miR-498后SCC-15细胞的增殖活性、划痕愈合率、侵袭细胞数目以及细胞周期蛋白D1、波形蛋白表达水平下调,miR-498和E-钙黏蛋白表达水平上调(P<0.05);抑制miR-498表达可减弱下调hsa_circ_0085576表达对OSCC细胞增殖、迁移及侵袭的抑制作用;上调BMI-1表达可减弱过表达miR-498对OSCC细胞增殖、迁移及侵袭的抑制作用。结论下调hsa_circ_0085576表达可通过激活miR-498/BMI-1轴抑制OSCC细胞增殖、迁移及侵袭。Objective This study aims to explore the effects of circular RNA hsa_circ_0085576 on cell migration and invasion of oral squamous cell carcinoma(OSCC)and the underlying molecular mechanism.Methods Circular RNA hsa_circ_0085576,microRNA-498(miR-498),and B-cell-specific Moloney murine leukemia virus integration site 1(BMI-1)in the cells of OSCC were detected by quantitative real-time polymerase chain reaction(qRT-PCR)and Western blot analyses.CCK-8,scratch test,Transwell test,qRT-PCR,and Western blot were used to detect the proliferation,migration,and invasion ability of SCC-15 cells and the expression of related genes and proteins.Results The expression of hsa_circ_0085576 and BMI-1 in the cells of OSCC was upregulated,and that of miR-498 was downregulated(P<0.05).The proliferative activity,scratch healing rate,number of invasive cells,and expression levels of cyclinD1 and vimentin proteins of SCC-15 cells were downregulated,whereas the expression levels of miR-498 and E-cadherin proteins were upregulated(P<0.05).Inhibition of miR-498 expression weakened the inhibitory effect of downregulating hsa_circ_0085576 on the proliferation,migration,and invasion of OSCC cells.Upregulation of BMI-1 expression attenuated the inhibitory effect of overexpressed miR-498 on the proliferation,migration,and invasion of OSCC cells.Conclusion Downregulation of hsa_circ_0085576 expression inhibited the proliferation,migration,and invasion of OSCC cells by activating the miR-498/BMI-1 axis.

关 键 词：环状RNA hsa_circ_0085576 微小RNA-498 B细胞特异性莫洛尼鼠白血病病毒整合位点1 口腔鳞状细胞癌 

分 类 号：R730.2[医药卫生—肿瘤]